FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363027909
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC16223230.44688657807849147Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7848750.032.069279
GAGCGTC8434750.029.8515268
AGAGCGT8891000.028.3463577
AAGAGCG9636600.026.2711876
CGGAAGA9722350.026.0660533
TCGGAAG9755100.025.9466652
ATCGGAA9807800.025.802131
GAAGAGC11780350.021.6682955
GGAAGAG14921250.017.3411314
CGCCGTA3834050.013.31367155-59
TCGCCGT3928300.013.03373855-59
GCCGTAT3952950.012.90000555-59
CGTATCA4097700.012.59991260-64
CCGTATC4120900.012.37531455-59
GTCGCCG4171750.012.297461555-59
TGGTCGC4433750.011.55828850-54
GTGGTCG4621950.011.18332650-54
GGTCGCC4593250.011.11526150-54
TCTCGGT4929600.010.59736145-49
TCGGTGG4891450.010.55462645-49
Produced by FastQC (version 0.11.5)