EGAF00003249957 FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content
Kmer Content

Basic Statistics

Measure	Value
Filename	EGAF00003249957
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340664859
Sequences flagged as poor quality	0
Sequence length	151
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	633375	0.18592319790753645	No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC	415310	0.12191160579905895	Illumina Single End PCR Primer 1 (96% over 31bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	631100	0.0	10.178603	9
GAGCGTC	681235	0.0	9.478466	8
AGAGCGT	729220	0.0	8.864808	7
AAGAGCG	796250	0.0	8.141393	6
CGGAAGA	817460	0.0	7.9137864	3
TCGGAAG	829650	0.0	7.797831	2
GCGTCGG	24280	0.0	7.671689	145
ATCGGAA	836910	0.0	7.663918	1
GAAGAGC	995900	0.0	6.724241	5
CGCCGTA	200720	0.0	6.663773	55-59
TCGCCGT	210725	0.0	6.40931	55-59
GCCGTAT	214900	0.0	6.278045	55-59
CGTATCA	225085	0.0	6.116918	60-64
CCGTATC	224305	0.0	5.970854	55-59
GTCGCCG	233235	0.0	5.8665757	55-59
GGAAGAG	1277675	0.0	5.459186	4
TGGTCGC	255255	0.0	5.3479676	50-54
GTGGTCG	264990	0.0	5.236854	50-54
GGTCGCC	261725	0.0	5.2091146	50-54