FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003249973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597307
Sequences flagged as poor quality0
Sequence length101-126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN538692.074032834778484No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC41130.15835632830466323No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC32390.12470608980763537No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC30820.11866136733162465No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC28950.11146160234427428No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA28230.10868950031705917No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG28090.10815048047843401No Hit
CTACAACATCCAGAAAGAGTCTACCCTGCACCTGGTGCTCCGTCTCAGAG27340.10526287420008493No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC27140.10449284585919184No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC26370.10152823674675346No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC26280.10118172399335157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)