FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003249977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699415
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT83100.48899180011945287No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC53460.3145788403656552No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT50260.29574883121544765No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC40920.24078874200827932No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC40030.23555164571337786No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC39990.23531627059900023No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA39510.2324917692264691No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA37910.22307676465136533No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC37870.22284138953698773No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC36560.2151328545411215No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC35010.20601206885898973No Hit
CCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCC34000.20006884722095544No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT33460.19689128317685792No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT30470.1792969933771327No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT30270.17812011780524473No Hit
TGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTA29750.175060241318336No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC29690.17470717864676963No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC29550.17388336574644803No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC29410.17305955284612648No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC27540.16205576624897391No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA27190.15999623399816995No Hit
GTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGC26200.1541706999173245No Hit
CTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT25850.15211116766652052No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT25370.1492866662939894No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG23720.13957744282591364No Hit
CCAGCACAACCTCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAACT23510.13834172347543125No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG23440.13792981702527046No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT23170.1363410350032217No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC22510.13245734561599137No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC22380.1316923764942642No Hit
CCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTC22110.13010359447221542No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA22010.12951515668627145No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT22000.12945631290767706No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT21870.12869134378594987No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG21280.12521956084888033No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG20420.12015899588976207No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT20410.12010015211116767No Hit
GAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACA19940.11733449451723094No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT19310.11362733646578381No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA18900.11121474154341347No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA18690.10997902219293108No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG18610.1095082719641759No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA18560.10921405307120391No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18390.10821370883509913No Hit
AACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAA18210.10715452082039996No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC17990.1058599576913232No Hit
CACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGGTCCTG17970.10574227013413438No Hit
GCACAACCTCTGCTCCTACAACTAGCACAACCTCTGGTCCTGGAACTACT17700.10415348811208563No Hit
ACCAGCACAACCTCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAAC17440.10262354986863126No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC17270.1016232056325265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)