FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003249978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251
Sequences flagged as poor quality0
Sequence length47-610
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTGACTACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA2071.839836458981424No Hit
CGACGTGACTACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA1871.662074482268243No Hit
CGACGTGACTACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1811.6087458892542885No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA1811.6087458892542885No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1761.5643053950759933No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1701.5109768020620389No Hit
CGACGTGACTACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA1621.4398720113767665No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1551.3776553195271533No Hit
CGACGTGACTACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT1531.359879121855835No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1531.359879121855835No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1511.342102924184517No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC1471.3065505288418808No Hit
CGACGTGACTACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA1461.2976624300062216No Hit
CGACGTGACTACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG1391.2354457381566084No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT1361.2087814416496312No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT1361.2087814416496312No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT1341.1910052439783132No Hit
CGACGTGACTACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA1341.1910052439783132No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT1321.173229046306995No Hit
CGACGTGACTACACTGACGACATGGTTCTACACGATGAACAATGACATCT1301.1554528486356768No Hit
CGACGTGACTACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC1271.1287885521286998No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA1261.1199004532930408No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT1261.1199004532930408No Hit
CGACGTGACTACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT1251.1110123544573816No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG1231.0932361567860636No Hit
CGACGTGACTACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG1231.0932361567860636No Hit
CGACGTGACTACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT1221.0843480579504043No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA1221.0843480579504043No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC1211.0754599591147453No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA1211.0754599591147453No Hit
CGACGTGACTACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA1201.0665718602790863No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG1181.0487956626077684No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC1131.004355168429473No Hit
CGACGTGACTACACTGACGACATGGTTCTACACCATGGACACTGGATTAA1120.9954670695938139No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT1120.9954670695938139No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT1110.9865789707581549No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT1110.9865789707581549No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA1100.9776908719224958No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT1080.9599146742511777No Hit
CGACGTGACTACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT1070.9510265754155187No Hit
CGACGTGACTACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT1060.9421384765798596No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT1050.9332503777442005No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA1050.9332503777442005No Hit
CGACGTGACTACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC1050.9332503777442005No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1040.9243622789085415No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC1030.9154741800728825No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA1030.9154741800728825No Hit
CGACGTGACTACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC1000.8888098835659053No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC980.8710336858945872No Hit
CGACGTGACTACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA970.8621455870589281No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC930.8265931917162919No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA920.8177050928806329No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA920.8177050928806329No Hit
CGACGTGACTACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG900.7999288952093148No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG890.7910407963736557No Hit
CGACGTGACTACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT880.7821526975379967No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT880.7821526975379967No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA870.7732645987023377No Hit
CGACGTGACTACACTGACGACATGGTTCTACAACAGGGATATTACCTACC860.7643764998666785No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA860.7643764998666785No Hit
CGACGTGACTACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC830.7377122033597014No Hit
CGACGTGACTACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT810.7199360056883832No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA790.7021598080170652No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA780.6932717091814061No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC750.666607412674429No Hit
CGACGTGACTACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA750.666607412674429No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG750.666607412674429No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT740.6577193138387699No Hit
CGACGTGACTACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG730.6488312150031108No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA720.6399431161674518No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA710.6310550173317928No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC710.6310550173317928No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT700.6221669184961337No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC700.6221669184961337No Hit
CGACGTGACTACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC690.6132788196604746No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT660.5866145231534975No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG660.5866145231534975No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT650.5777264243178384No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG640.5688383254821794No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG600.5332859301395432No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG560.49773353479690696No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA510.4532930406186117No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG500.44440494178295264No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT490.4355168429472936No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATAATC480.42662874411163454No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT470.4177406452759755No Hit
CGACGTGACTACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC440.3910763487689983No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC430.3821882499333393No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT390.34663585459070306No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC380.337747755755044No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT380.337747755755044No Hit
CGACGTGACTACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC370.32885965691938496No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT320.2844191627410897No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA300.2666429650697716No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG260.23109056972713538No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT260.23109056972713538No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCAAACCAGGATTCTAGC220.19553817438449916No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA220.19553817438449916No Hit
CGACGTGACTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTGG220.19553817438449916No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC210.1866500755488401No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT210.1866500755488401No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC210.1866500755488401No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT210.1866500755488401No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT190.168873877877522No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA190.168873877877522No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCTTTCCTCTGCCGTGTTT180.15998577904186295No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCCTTATTTCCCCCCATT170.1510976802062039No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG170.1510976802062039No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCAGGACCCATTAGAACCA160.14220958137054485No Hit
CGACGTGACTACACTGACGACATGGTTCTACACCATTTATGAATCTAAAG150.1333214825348858No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCAGCCCACCCTGGATTAA150.1333214825348858No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGGGAAGATT150.1333214825348858No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGGAAATGCTCTTATTAT150.1333214825348858No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGACTTTT140.12443338369922674No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC130.11554528486356769No Hit
CGACGTGACTACACTGACGACATGGTTCTACAGTAGACCAAGTCTCTGCTACCGTAAGTCACGTCG120.10665718602790863No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTCGTGCCTTCCCATTGCCT120.10665718602790863No Hit
CGACGTGACTTACGGTAGCAGAGACTTGGTCTACTGTAGAACCATGTCGTCAGTGTAGTCACGTCG120.10665718602790863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)