FastQCFastQC Report
Mon 6 Mar 2023
EGAF00003250182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137432681
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC21435021.5596741505755825RNA PCR Primer, Index 10 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG4581660.33337485426774144RNA PCR Primer, Index 10 (100% over 49bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG2102800.15300581962742907RNA PCR Primer, Index 10 (100% over 49bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG1634310.11891713005293114RNA PCR Primer, Index 10 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)