FastQCFastQC Report
Thu 9 Mar 2023
EGAF00003250183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132726482
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC128865779.70912270544472RNA PCR Primer, Index 11 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG26187171.973017713224705RNA PCR Primer, Index 11 (100% over 49bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG11413880.8599549862249796RNA PCR Primer, Index 11 (100% over 49bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG9927490.7479660313757129RNA PCR Primer, Index 11 (100% over 49bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG4715020.3552433492511314RNA PCR Primer, Index 11 (100% over 49bp)
GCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT1911880.14404661158727916RNA PCR Primer, Index 11 (100% over 46bp)
CCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT1742200.13126242583601366RNA PCR Primer, Index 11 (100% over 46bp)
CTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTAT1434430.10807413700605732RNA PCR Primer, Index 11 (100% over 48bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC1364140.1027782835380207RNA PCR Primer, Index 11 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)