FastQCFastQC Report
Mon 6 Mar 2023
EGAF00003250184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134451256
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC83081886.179330894461856RNA PCR Primer, Index 12 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG23648711.758905844657933RNA PCR Primer, Index 12 (100% over 49bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG9201380.6843654922792242RNA PCR Primer, Index 12 (100% over 49bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG6556820.48767264769917806RNA PCR Primer, Index 12 (100% over 49bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG5340240.39718781057723995RNA PCR Primer, Index 12 (100% over 49bp)
GATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTAT1827860.1359496411100838RNA PCR Primer, Index 12 (100% over 48bp)
GCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT1725870.12836399237505078RNA PCR Primer, Index 12 (100% over 46bp)
CTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTAT1541660.11466311627464454RNA PCR Primer, Index 12 (100% over 48bp)
GCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTAT1394430.10371267933711234RNA PCR Primer, Index 12 (100% over 48bp)
CCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT1364060.10145386815873256RNA PCR Primer, Index 12 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)