FastQCFastQC Report
Thu 16 Mar 2023
EGAF00003250185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112566490
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC42918943.812763460955387RNA PCR Primer, Index 4 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG5949820.5285604978888477RNA PCR Primer, Index 4 (100% over 49bp)
GCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC3561120.3163570259674971RNA PCR Primer, Index 4 (100% over 44bp)
CCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC3412040.30311329774962337RNA PCR Primer, Index 4 (100% over 44bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG2244450.1993888234411502RNA PCR Primer, Index 4 (100% over 49bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG2100600.18660970951479433RNA PCR Primer, Index 4 (100% over 49bp)
CTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1941750.1724980498192668RNA PCR Primer, Index 4 (100% over 44bp)
CCGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1792070.15920101977062623RNA PCR Primer, Index 4 (100% over 44bp)
ACCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1708340.1517627492871102RNA PCR Primer, Index 4 (100% over 44bp)
CCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1555930.1382231959084804RNA PCR Primer, Index 4 (100% over 44bp)
CCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1255940.11157316888889403RNA PCR Primer, Index 4 (100% over 44bp)
GCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1139490.10122817190089164RNA PCR Primer, Index 4 (100% over 44bp)
CCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC1139120.10119530243858542RNA PCR Primer, Index 4 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)