FastQCFastQC Report
Wed 15 Mar 2023
EGAF00003250186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102524250
Sequences flagged as poor quality0
Sequence length85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC13726911.338893968987825RNA PCR Primer, Index 3 (100% over 50bp)
GCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC1692110.16504485524156481RNA PCR Primer, Index 3 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG1619140.1579275147099345RNA PCR Primer, Index 3 (100% over 49bp)
CCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC1579060.15401819569516478RNA PCR Primer, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)