FastQCFastQC Report
Tue 26 Sep 2023
EGAF00003250191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54392427
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC35457766.518878078376609RNA PCR Primer, Index 2 (100% over 50bp)
GCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC7564291.3906880823685253RNA PCR Primer, Index 2 (100% over 44bp)
CCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC6411241.1787008511313533RNA PCR Primer, Index 2 (100% over 44bp)
CCGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC3463820.6368202691157723RNA PCR Primer, Index 2 (100% over 44bp)
ACCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC3073030.5649738703514737RNA PCR Primer, Index 2 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG2958940.5439985239121615RNA PCR Primer, Index 2 (100% over 49bp)
CTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC2384330.4383569793640575RNA PCR Primer, Index 2 (100% over 44bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG1499630.27570566027509674RNA PCR Primer, Index 2 (100% over 49bp)
CCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1454680.26744164219772726RNA PCR Primer, Index 2 (100% over 44bp)
TTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1263910.23236874500930063RNA PCR Primer, Index 2 (100% over 44bp)
CCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1179230.21680040127644976RNA PCR Primer, Index 2 (100% over 44bp)
CCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1067780.19631041652177056RNA PCR Primer, Index 2 (100% over 44bp)
GCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1044900.19210394858828417RNA PCR Primer, Index 2 (100% over 44bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG1036810.19061660918348064RNA PCR Primer, Index 2 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCGCGTATGC1034340.19016250184975197RNA PCR Primer, Index 2 (98% over 50bp)
ACCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC982460.18062440935022075RNA PCR Primer, Index 2 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATTC967290.1778354181548104RNA PCR Primer, Index 2 (98% over 50bp)
GGCACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC900160.16549362653003147RNA PCR Primer, Index 2 (100% over 44bp)
CACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC866410.1592887186298931RNA PCR Primer, Index 2 (100% over 44bp)
ACCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC710020.1305365542890741RNA PCR Primer, Index 2 (100% over 44bp)
GCACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC698930.12849766751536937RNA PCR Primer, Index 2 (100% over 44bp)
GGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC581640.10693400388256255RNA PCR Primer, Index 2 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)