FastQCFastQC Report
Fri 2 Jun 2023
EGAF00003250193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97440841
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC21708662.2278810175704455RNA PCR Primer, Index 1 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG3833740.39344282753060394RNA PCR Primer, Index 1 (100% over 49bp)
CCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1833790.1881952147765227RNA PCR Primer, Index 1 (100% over 44bp)
CCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1367410.14033232738621376RNA PCR Primer, Index 1 (100% over 44bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG1222710.12548229135255512RNA PCR Primer, Index 1 (100% over 49bp)
CCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1186110.12172616613602502RNA PCR Primer, Index 1 (100% over 44bp)
CCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1098240.1127083868251917RNA PCR Primer, Index 1 (100% over 44bp)
GCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1063390.10913185775972521RNA PCR Primer, Index 1 (100% over 44bp)
CTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1061220.10890915853240635RNA PCR Primer, Index 1 (100% over 44bp)
CCGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1007930.10344019916658971RNA PCR Primer, Index 1 (100% over 44bp)
GCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC982160.10079551755921318RNA PCR Primer, Index 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)