FastQCFastQC Report
Tue 26 Sep 2023
EGAF00003250198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61241836
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2622960.42829545476069664Illumina Single End PCR Primer 1 (100% over 50bp)
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG1062310.1734614879932731No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1057480.17267281144216512No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1000740.16340790305502925No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT837700.13678557906069308No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG704210.11498838800325974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)