FastQCFastQC Report
Tue 5 Sep 2023
EGAF00003250200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003250200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80576352
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3931830.4879632674360835Illumina Single End PCR Primer 1 (100% over 50bp)
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1817440.22555501147532714No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1153310.14313256574335853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)