FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003287448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003287448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417290818
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG14428640.3457694101479127TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3205850.083.618721
TCGGAAG3328300.080.348652
CGGAAGA3513300.076.095383
AGAGCAC5828400.045.9228027
AGCACAC6062100.044.016389
GAGCACA6218450.043.0180558
GAAGAGC6444950.041.7578475
AAGAGCA8838550.030.6640876
GGAAGAG9911200.027.5328484
TATGCCG2304500.023.7088145-49
CCCGATC2405850.022.52573235-39
CGTCCCG2370950.022.1562930-34
CGTATGC2456200.022.02079245-49
CTCGTAT2201050.022.0110140-44
TCCCGAT2460950.021.94715335-39
GTCCCGA2478950.021.65502535-39
CCGATCT2215900.021.6339935-39
TCTCGTA2240900.021.55682440-44
ATGCCGT2553250.021.29790945-49
GCCGTCT2572200.020.7826650-54
Produced by FastQC (version 0.11.5)