FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003287449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003287449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417290818
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT12922360.30967276159908225Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2578550.089.6987849
GAGCGTC2899000.079.728988
ATCGGAA3294850.076.053921
TCGGAAG3422850.072.767182
AGAGCGT3206200.072.3417747
CGGAAGA3637700.068.416273
AAGAGCG3706350.066.4801946
AGCGGCG621950.039.723959
GAAGAGC6479150.038.4466675
GAGCGGC859100.028.2505478
ATCGGGA590150.025.1168161
GGAAGAG10285200.024.739964
CGCCGTA1919300.023.9541145-49
GTCGCCG2146700.022.4936940-44
AGAGCGG1117000.022.3107327
TCGCCGT1962000.022.05990640-44
GCCGTAT2155700.021.5551245-49
GGTCGCC2315750.020.93363240-44
TGGTCGC2268000.020.88808840-44
CCGTATC2265850.020.58400745-49
Produced by FastQC (version 0.11.5)