FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003287450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003287450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418719105
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1499200.045.2198371
TCGGAAG1605700.042.2389342
CGGAAGA1800300.037.664313
TATGCCG814650.017.17850545-49
AGAGCAC4165400.016.870177
AGCACAC4432150.015.791079
GAGCACA4562050.015.4589818
GAAGAGC4743400.014.9382015
CGTATGC949750.014.63414245-49
CTCGTAT885300.014.41337440-44
TCTCGTA921450.013.78182940-44
ATGCCGT1073200.013.17504645-49
TGCCGTC1051000.012.9608445-49
GCCGTCT1097750.012.73889850-54
TCGTATG1003250.012.36765140-44
ATCTCGT1140300.011.29189640-44
CGTCTTC1368100.010.53314350-54
CGTCTGA1377900.010.34811215-19
CCGTCTT1394600.010.29660650-54
AAGAGCA7156250.010.2701346
Produced by FastQC (version 0.11.5)