FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003287841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003287841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424661924
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT12923590.30432655412732507Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2540800.089.281549
GAGCGTC2856700.079.354448
ATCGGAA3286300.074.726061
AGAGCGT3168000.071.8413547
TCGGAAG3400150.071.820012
CGGAAGA3608300.067.5827563
AAGAGCG3667800.065.797166
AGCGGCG613550.037.5587969
GAAGAGC6546700.037.3373575
GAGCGGC857850.026.668958
GGAAGAG10385050.024.078534
CGCCGTA1876100.023.99836745-49
GTCGCCG2115250.022.39834840-44
TCGCCGT1939650.022.03612740-44
ATCGGGA589800.021.5877481
GCCGTAT2107700.021.57875645-49
AGAGCGG1096650.021.2519257
TGGTCGC2227800.020.85150540-44
GGTCGCC2287200.020.79560540-44
CCGTATC2218600.020.59161245-49
Produced by FastQC (version 0.11.5)