FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003289728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003289728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGACAGTAGCTCAAAAGAGGAGAGGCTCCCGACATATGTTCTCTAGG62.666666666666667No Hit
GTGAAAGTAGCTCAGGTTATCTGTCACCCCACCACATCCCACCCACCCTG41.7777777777777777No Hit
GACCTCGTAGCTCACCTGGCTGAAGGCGAGCGACAGCTTCATCCCCAGGA41.7777777777777777No Hit
AAACTTAGTAGCTCACCGCCTAAATTCATATGACGTTTATTTGCTCTTTG41.7777777777777777No Hit
GGCTCCGTAGCTCATGTGATGTTTGCATTCAACTCACAGAGTTGAACCTT31.3333333333333335No Hit
GGTCTTAGTAGCTCACATTGATATCAAATACCCTGACCTCGGCCCGGAAG31.3333333333333335No Hit
AAATAAGTAGCTCATCTGCAACCAGGATACGCTGGTGCCCTATGGGAACC31.3333333333333335No Hit
TACATGCAGTAGCTCACGTGAGATTTGATGCTAGGGAAGGAGGCATGGGG31.3333333333333335No Hit
GCGCGGCAGTAGCTCACGGCATTGGAGCCCTCATCGGCATCACTTGCAAG31.3333333333333335No Hit
GCGCGCCAGTAGCTCACTGGAGCCCCCAGGCAGGACCCACAGCCCCTCTG31.3333333333333335No Hit
AATCTGGTAGCTCAATCCATGCACGCTTTGGGAATCTCAGCCTCTACCTG31.3333333333333335No Hit
TCAGTAAGTAGCTCACGCCGCAGGAGGAGCAGCGGAAGGGCCGGCCGCCC31.3333333333333335No Hit
TATGCAAGTAGCTCAGCTGAGGGCAAACATCGAGGAACTGCGCTGCGCCA31.3333333333333335No Hit
AAATGTTCAGTAGCTCATCTCAGCAAGAAAGTCGATGTCTCGGGAGGCGA20.8888888888888888No Hit
ATATCACAGTAGCTCAGGGTGTGTATGCGGGGCCAGGGTGGAAGCCTGCA20.8888888888888888No Hit
TGGGGTTCAGTAGCTCAATAAAATATCTTTTTCTGGGGGGACTAGGTGGG20.8888888888888888No Hit
GATCGGGTAGCTCACCCACCTGGAGGAGGAGGTTGGAGGGCTGCCCTGCA20.8888888888888888No Hit
AATACCTCAGTAGCTCACCCTCTGTAATTGCACGTGTCTTCATGACAGCA20.8888888888888888No Hit
GGTCTGGTAGCTCACCAGGATGCGGTCGTACACGGCCTTGGTGGACTGCA20.8888888888888888No Hit
TGACCTGTAGCTCAACAACCCCATCTTTGCACAAGCTTTGTATAAAGTGG20.8888888888888888No Hit
AACCGCTCAGTAGCTCACCACGGACTTCCAGGAGATCTTTCCCATGGGTG20.8888888888888888No Hit
CGTGGGAGTAGCTCACCGTGACCCCAACCCCAACACCCACCGGCACACAG20.8888888888888888No Hit
ATAAGAGTAGCTCACCCTGGTGGCCAGGATCACCCAGAATCCCGCGTTTG20.8888888888888888No Hit
CAGGTAAGTAGCTCACCAAGGTTTGGGATGATCAGGCTGTATGGAATGTG20.8888888888888888No Hit
GCGATTTCAGTAGCTCACTAGGAAGGGTTCTTCTTCCTCTGGTAGACTGT20.8888888888888888No Hit
CAAAAGCAGTAGCTCATGACAAAGAGGGAATAGCGAAAAACCGCCTGGAA20.8888888888888888No Hit
AGGAACGTAGCTCACCAGGCTCCCAGCACAGCGCACGGCCCTCACAGTGA20.8888888888888888No Hit
TCATGCGTAGCTCACCTGACCTCAAGTGATCCTCTCTACTCAGCCTCCCA20.8888888888888888No Hit
AGACTCTCAGTAGCTCACACATAATAATGGATAGCATACCTCCTTCTGGC20.8888888888888888No Hit
ACTCATTCAGTAGCTCAGGCTCTCGATCTCCATCCTCTCCTTTAACCTTC20.8888888888888888No Hit
TCGCTCAGTAGCTCAGGCTTGGGGGCACCCAGTGGTGTGGGCCTGACCCC20.8888888888888888No Hit
TGGGCCGTAGCTCATATTCGCCTTCGAAGAGGCTGGAGTCGTGCCCTGAG20.8888888888888888No Hit
CCCGAACAGTAGCTCAGGCGTTGTGCTGTGGACCGAGGGGACCCCATCAC20.8888888888888888No Hit
GAAGGTTCAGTAGCTCATACAAAGCTGTTTACACACAGGAAGGGGCAGGC20.8888888888888888No Hit
ACCGCACAGTAGCTCACATCATTTGGAGTGGGACGCCGAGCGTAAGTTCT20.8888888888888888No Hit
GTACTGGTAGCTCAGCCCCCACAGGAGCCACGGCCCCCCTTGGAGCCCCC20.8888888888888888No Hit
CGACTAGTAGCTCAGCTGCTAGGGGCTGGGGGCTGCAGAGACAACAGCAG20.8888888888888888No Hit
GGGCATTCAGTAGCTCACGCTGAACCAGTTGGACTCTCACGTTCTGCTGT10.4444444444444444No Hit
AGCCTCTCAGTAGCTCAGGCCGAAGGCAGCGAGCCGCAGTGCGTGCTGGA10.4444444444444444No Hit
CAAGATGTAGCTCACTTTTCACAGTACGCAGGCTGAGTAAAACCCGCCCC10.4444444444444444No Hit
GGTGGTAGTAGCTCACCACAAAATCTAATAGATGTCTTTGTATTCCTCCT10.4444444444444444No Hit
AGAAACTCAGTAGCTCAATTTGTGGGAACTGAATAAAAATCATATGTTTG10.4444444444444444No Hit
CGCTTAAGTAGCTCAACAAAGGTCCCTGCACTCCAGGGCCCAGGGGATTG10.4444444444444444No Hit
CAGTGTAGTAGCTCATAACTCCAGTCCTTGCAAAAACACAAAGTTCTGGT10.4444444444444444No Hit
ATATCACAGTAGCTCAGGGTGTGTATGTGGGGCCAGGGTGGAAGCCTGCA10.4444444444444444No Hit
ACATTTCAGTAGCTCACCCTGAACCAGGAGTCTCCAGATGTCAACACAGA10.4444444444444444No Hit
GCCAATAGTAGCTCACCAGCCTTCTGGAGTGACAGATGGCATGAGTGAGC10.4444444444444444No Hit
CAAACATCAGTAGCTCATAGATTCTCTGGTCATTGAGCATATCCAAGTGA10.4444444444444444No Hit
TTTTGTCAGTAGCTCAGGTGGATATAATAGATTTGGACGTGACGGTGGCA10.4444444444444444No Hit
CGCCGGTCAGTAGCTCACACCAGTCTCCAGAGATACATCTTTAACTGGAA10.4444444444444444No Hit
GGCAATTCAGTAGCTCAACTGGTAACACGTGAGGATTTTCATAGACAATT10.4444444444444444No Hit
CTAAATGTAGCTCACAACACACTTGTGTTCATTGTGAATGTTAAAGCCAA10.4444444444444444No Hit
TGGGAACAGTAGCTCAGGCCCACTTGGGCCGGACGTCAGCCTCACTTGCC10.4444444444444444No Hit
AAAAACGTAGCTCATGTGAGCAAAGAAATCACGTTACTTCTTCCAGCTGA10.4444444444444444No Hit
GCGCCCTCAGTAGCTCATGGCCCACAGGCTGCCCACCACTGGCTACCTCG10.4444444444444444No Hit
CAGCCTTCAGTAGCTCACGTCTTCGGACGAGGAGTGCTTCTTTGACCTGT10.4444444444444444No Hit
CCAAGTTCAGTAGCTCACCCTGTCACCAGCCCGAAGGACAGTACGCACAA10.4444444444444444No Hit
ACTTCTAGTAGCTCACCAAGATATTCCCCATCCATCTTCTTAATAGCTTT10.4444444444444444No Hit
AGTCAATCAGTAGCTCATGAGTAATAGTGGCTGGGCGCAGTTGCTCACGC10.4444444444444444No Hit
GCGACTGTAGCTCACGGGGTCAGAGAGCCAACAGCCTACTCTGACTCAAG10.4444444444444444No Hit
TCGCCGGTAGCTCACGTGGGCCTCAGTCTCAAGGAGCAGGGTGAGTGTCT10.4444444444444444No Hit
TGAAGGAGTAGCTCACACAAAGTGTGCTTCTCTGGGTGGAGCAGGCTGGC10.4444444444444444No Hit
TATTATAGTAGCTCACAGTTGTTTTAAAAATAGCCCAAACAAGCAGATTT10.4444444444444444No Hit
AAAAATCAGTAGCTCACTTTCATGTTGGATAGTGCTGCTATGATAACAGT10.4444444444444444No Hit
TAGATGTCAGTAGCTCACTGGCCAGAAGGCTATGCTGAAGTTTGCTGCTG10.4444444444444444No Hit
GAAAACAGTAGCTCAGCCGGCTCCTGTCCCTCCCACCCCAGGGCCCTGGG10.4444444444444444No Hit
ATCGGCCAGTAGCTCATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTA10.4444444444444444No Hit
TGAGCGAGTAGCTCACATTCCTCACAACGGCCTTCCCGGCCCATTTTTTC10.4444444444444444No Hit
TATTTCAGTAGCTCATGAGGTTTGAACCCCTGAGGTGCTGCACAAACTTG10.4444444444444444No Hit
ACAGAAAGTAGCTCATGAATTTTTTACATCTGCCTGGGTCCAATGTGCTG10.4444444444444444No Hit
CCCTAGTCAGTAGCTCATTAGCAAAGATGAACCTGCAGCAGGATGGGAAA10.4444444444444444No Hit
AACTAAGTAGCTCATTGTGGACGAAGGTCACCGCATGAAGAACCACCACT10.4444444444444444No Hit
ATGGAGAGTAGCTCACAAGAAAGTGAAGACTATTCTCAGCCATCATCTTC10.4444444444444444No Hit
ACCCGCCAGTAGCTCAGCAGATCCTTCGTACCGGGAGGGACCTCTTCAGC10.4444444444444444No Hit
AACTACAGTAGCTCATCACATTTGTAAGGTTTCTCTCCAGTATGAAGTCT10.4444444444444444No Hit
CTGTGTAGTAGCTCAGCCCTTCAAGACATGGACAAATTTAGCTTGAAAGA10.4444444444444444No Hit
TGTGGGGTAGCTCACGTGTCAGTAGCTCACCCACCTGCGGAAGGCAGGCA10.4444444444444444No Hit
AGCGCGGTAGCTCACCCAGCTCTGCCTGCCCTACCTTGGCCCAGCTGAAT10.4444444444444444No Hit
ACTATTAGTAGCTCAATCCATGGCAAATGCTGGACCCAACACAAATGGTT10.4444444444444444No Hit
CGGGCGCAGTAGCTCATGGATCGTGTGGTGTGCAAAGGAAAAAGTTGCAA10.4444444444444444No Hit
CGGCTTAGTAGCTCACCCTTCTCTGTTCCAGGCCCTGGTCAAATTCCTGA10.4444444444444444No Hit
CGCTGCAGTAGCTCACAGCTAAAAGCATATTGACTGATGAGCAAGAAAGA10.4444444444444444No Hit
TAAATACAGTAGCTCAGACTTGCCCGGCAGGTGATGGTGACTCTGTCTCC10.4444444444444444No Hit
CTTTTAGTAGCTCATATGCTGCTGTTTTGTTATGTGGAGGTTTTTCTAAA10.4444444444444444No Hit
GCGGCCAGTAGCTCACGATGACTGGGTAGTGCAGAGGGCGACAGATGGCT10.4444444444444444No Hit
AGGAATCAGTAGCTCATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGT10.4444444444444444No Hit
GGAAGGTAGTAGCTCAGGTGCTACATCAGGTGTTGTGATAGACATGGGGA10.4444444444444444No Hit
TCTGTGAGTAGCTCAAGGAGAGTTGTAAGACTGAGGTCCTGCCTGCCAGG10.4444444444444444No Hit
CCGCCTGTAGCTCATCTGCTTTAGGACTCACTTTATTAAACCTTCTTCAA10.4444444444444444No Hit
CGAAGCGTAGCTCACCTTACTCCAAGCTTCCCACTCCAGACCTACTTTGG10.4444444444444444No Hit
GATAAACAGTAGCTCACCCCCGCCCGGGGGTGGTCCCAAGGGGCCCACTG10.4444444444444444No Hit
CGACAGAGTAGCTCATAACTGTACGAGATTTCGAGAACATCGGAGAATAC10.4444444444444444No Hit
GCTCTCCAGTAGCTCATCCCGGACCAGATCGTGCCGCAAACGAAAGGCCA10.4444444444444444No Hit
TAATGTCAGTAGCTCAGGCGGGGGTGGGGTGGTGAATAGGCATGAGAGCT10.4444444444444444No Hit
TAGGTACAGTAGCTCAGAATACAAAACCTTGTCTTTGTTCTGTCACATCA10.4444444444444444No Hit
TCGCTCAGTAGCTCAAGTAGTGCATGTAATCTGCAACATTCTGGCCCATG10.4444444444444444No Hit
GTCGTCGTAGCTCACCCCAGAGTTCCAGGCCAAGAGGTGCAGAAGGTCAA10.4444444444444444No Hit
TGCATCTCAGTAGCTCACCCACATTGCTTACACCCATCGGGTTTCTCTCC10.4444444444444444No Hit
TAATACTCAGTAGCTCAAGGGACAATTGATTCAGGGGTCTTGACAGAGGA10.4444444444444444No Hit
TGTGGCCAGTAGCTCACCAGCCAGTGCCCAGGGCTCTCCAGCCAGTGCCC10.4444444444444444No Hit
AAAGGGTCAGTAGCTCAAAGCAATGGCAGGTGGGTTAACACAAGATGCCT10.4444444444444444No Hit
GTACTGGTAGCTCAGCCCCCACAGGCGCCACGGCCCCCCTTGGAGCCCCC10.4444444444444444No Hit
ACGTAAGTAGCTCAGGAGACATATTACACAGGTGAGTGTGTACCTAGATT10.4444444444444444No Hit
AAATTACAGTAGCTCAGGCACAGGAGATAGTCCATGATTTGCAGGGACAG10.4444444444444444No Hit
GATCTTGTAGCTCAGACAGGAGGTTCCACAGTGGAAGAGTGGGTGGGAAG10.4444444444444444No Hit
CGAGCGTCAGTAGCTCAGAAGGATGTGTCCAGCTCATCGGGGAAATCAGT10.4444444444444444No Hit
CGGGCGCAGTAGCTCATGGATCGTGTGGTTTGCAAAGGAAAAAGTTGCAA10.4444444444444444No Hit
CAAAGTGTAGCTCACCATCTCCATATGGATGTGCTTGAAGGTAACACCCT10.4444444444444444No Hit
GCTGAGTCAGTAGCTCAAACTCTGACTTTGTTGGTGCCGATTGCAGATCC10.4444444444444444No Hit
AGGTACTCAGTAGCTCAATAAAATTGCCAGATCCACGTCCATGACCCCTC10.4444444444444444No Hit
CTCATTTCAGTAGCTCACCAGTGAAACTCCTCTGAATGGTACACATTACT10.4444444444444444No Hit
GACAGTGTAGCTCACCCGCTGCCCTCCGCCGAGCCAGGCCTGCCTGGGAC10.4444444444444444No Hit
CTCAACCGTAGCTCACGAGGGGTCAGGCCCTGCTTGGGCAGCTGCCTTTT10.4444444444444444No Hit
GCCGGACAGTAGCTCATGTTGAGCTTCACAAAGGTTCCATTGAAGATTTG10.4444444444444444No Hit
TGCATCTCAGTAGCTCACCCACATTGCTTACACCCATCGGGTTTCACTCC10.4444444444444444No Hit
TGCAACCAGTAGCTCACCGCGTGGCGCGCGCCTCTAGCCGCCCTTCCCCT10.4444444444444444No Hit
TCGAGAGTAGCTCAGGAGCATGCCTTCTATACATGCAAAAAGGATGAAGA10.4444444444444444No Hit
AAATACTCAGTAGCTCAATGTATGAGCAGTGATGTTTATGTCAGGATTCT10.4444444444444444No Hit
ACGGCCGTAGCTCAGATGAATGACTAAATGGACGTTATTCTGAGTTTGTT10.4444444444444444No Hit
TGCATGAGTAGCTCATGAAAACTCTTATGATATCTGTAATAGAATTGAAT10.4444444444444444No Hit
CTATAACAGTAGCTCAAGCCTTCTTAACTTTTCTATGCTTTCTTTGATCT10.4444444444444444No Hit
CAGTTAGTAGCTCATCCCAAGGAATGATTTTCTTAAGGAGATAAAGATCA10.4444444444444444No Hit
GACGCCTCAGTAGCTCACCAGCAACATTGCCATGATGAACCTCCTTGACA10.4444444444444444No Hit
ATCGAGAGTAGCTCATGCACACAGACTCCTGCGAGTTCCCCTAAGTTCTT10.4444444444444444No Hit
TGGCGCCAGTAGCTCATGTTGAACCTGTTTAGATCATATGGAAGTTCCTG10.4444444444444444No Hit
CTGATCCAGTAGCTCATCCAAAAACTAGATGACAAGAATATTCTTTGTAT10.4444444444444444No Hit
AATAATCAGTAGCTCAAAAGTGCTCCATGGCCTCCACAATATTCCTGCCT10.4444444444444444No Hit
AGGCGTGTAGCTCAAGTGGGAGGAGACTTTGCAAATAGCAATCTTGGGGC10.4444444444444444No Hit
GCCGGGTCAGTAGCTCACTTGTTTTCTGCACAAGGTTTTGTCAAGAAAGA10.4444444444444444No Hit
ATAAGATCAGTAGCTCAGATGTTGGGCCAGGAGGACCGGCGTGTGCCCTT10.4444444444444444No Hit
TTATTCGTAGCTCACACTGAGGGCTACAATGGCACCACATTAACATAACA10.4444444444444444No Hit
ATAGTGCAGTAGCTCACAATGAGGTTTCTGAAAATTCTTCTGTCTAGGGT10.4444444444444444No Hit
ATACAACAGTAGCTCATCTCCATTGTGAAGACTGAGAGTTTGGATGGCCA10.4444444444444444No Hit
CTGTTTAGTAGCTCACGTCCGCTTCTCAGTGCACCGCGTGTCTTTCCATC10.4444444444444444No Hit
GAAGACCAGTAGCTCAGCCAGTTCCTGGACCACGACCTCGACTCCACGGT10.4444444444444444No Hit
GTATCCAGTAGCTCAATGGGAAAAAGTGGTTCAACGGTTGGTAGAGAGAG10.4444444444444444No Hit
CTACTCTCAGTAGCTCAACCTTCCGAGCGTCAGCTCACTCCCCTTCCACC10.4444444444444444No Hit
GATCACCAGTAGCTCATCGGTGCCATTAGGGCGTGTGAAGTCACCACCCT10.4444444444444444No Hit
AACTTCAGTAGCTCAATGGCATCTCCCCCAAATGCACCGCCTCGAAGAAA10.4444444444444444No Hit
TGAGACTCAGTAGCTCATACACAAAATGCCATGTTTGTTTAAGAAGAAGA10.4444444444444444No Hit
GCATCTTCAGTAGCTCAGAATGGAATGGAATCATCATCGAATGGACTCAA10.4444444444444444No Hit
ATAAATAGTAGCTCAACAAAACAAGGCTTTGCTCTATGGATTTTGTGACC10.4444444444444444No Hit
TAGTCATCAGTAGCTCAGGCGGAATTTTCTACAAAAGGATCCATGGCAGA10.4444444444444444No Hit
CTGATGGTAGCTCAGCAGGAGTGTGTGGTTTCAGCGCAGCATGGCTGTGG10.4444444444444444No Hit
CTATGGGTAGCTCATGGCCGATGTCCACCCTCTCCGGGCGTCCCTTCTCT10.4444444444444444No Hit
GTCCGCGTAGCTCATGTCGGTTGGGGCTGCAGTTTGCTAAGTTGGAGTAA10.4444444444444444No Hit
AATGAAGTAGCTCACGTGTGTCCCGAGCTCCTGTGGTTTCAGGTGGCCGC10.4444444444444444No Hit
TAGTACGTAGCTCAACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCG10.4444444444444444No Hit
TGTGCATCAGTAGCTCATGTGCTTCTCTTTTGTTTAGGGACCTCCAAGAT10.4444444444444444No Hit
GAACTTTCAGTAGCTCACCACCAGGGGTGGGCGGCACGGAGGTGCTCGAT10.4444444444444444No Hit
AAGTTCAGTAGCTCATCAGGAAACTATCTCAGCTCTTAGAGTGCTTTGTG10.4444444444444444No Hit
GTAGGGTCAGTAGCTCACCCCACCCCTATCTCCCTTCCCTGACTCTCTTT10.4444444444444444No Hit
CATGAAGTAGCTCAGGAAGATGGCCCTAAATTCTTGAAGTCTGGTGATGC10.4444444444444444No Hit
CCTTATTCATTAGCTCACTGCTCCACCCAGAGAAACACACTTTGTGAGAA10.4444444444444444No Hit
AAGCGTCAGTAGCTCATAATTGCTTATCAATAGAAATACCAACATGAATG10.4444444444444444No Hit
TCCTAATCAGTAGCTCATTTATGTAGAGCGATAGAGATACTATAGCTTCC10.4444444444444444No Hit
ACACAATCAGTAGCTCATTTGAGTTGTGAACTCACCTGCTTTTTCTCCCA10.4444444444444444No Hit
AACTCTTCAGTAGCTCACTTTTTTTTAAAGGAAAATTTGTATTACTTTAA10.4444444444444444No Hit
TTAGCATCAGTAGCTCATTTGGTCGGCTGACTGGGACCTTCCTGAGGAAT10.4444444444444444No Hit
GCAAAGAGTAGCTCACCTGAAGCCGATCAGCCTGTCAGAGTCACCCCGCC10.4444444444444444No Hit
CGCATTGTAGCTCACAGCAACTCAGGAGGCTGAGGGAGGAGAATCACTTG10.4444444444444444No Hit
TATGGAGTAGCTCATCTAGAGCCTTGGCCTTTTCTTTAGGAGTTTGTGGC10.4444444444444444No Hit
GAAAACGTAGCTCAGGTCAAAGCAGGAGGATCACTTGAACCTGGGAGGCG10.4444444444444444No Hit
ATGTACAGTAGCTCATGAAGACTGCTGGTAAAGACAGCATCTGCTTCCCT10.4444444444444444No Hit
GTGTCGCAGTAGCTCATAAAGGAAAACCCAGTTTAAGATTTCTCCAGGCC10.4444444444444444No Hit
CAGTCTGTAGCTCAGGCTTTCTTGGAATCGAATGGACGATTTTGAGCCTC10.4444444444444444No Hit
TGCTGACGTAGCTCAGTTAAGACCAGTACTTGTCCAGCACCTATTGGTTC10.4444444444444444No Hit
GCTCTCAGTAGCTCATATTAGTTTTACTTTTGGTTCTTCTGTTTGGGATT10.4444444444444444No Hit
TATTGTAGTAGCTCAAGGAATCATGTAGGAATGATTCCAGTTGCTTAAAC10.4444444444444444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)