FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003289734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003289734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCCAGTAGCTCACTGGAGCCCCCAGGCAGGACCCACAGCCCCTCTG72.9787234042553195No Hit
GATACCTCAGTAGCTCATCCTTACTCGTATGGGCACCACCCAATCTCCTC62.553191489361702No Hit
GTGGGGTCAGTAGCTCACATGCTTCCACTTCATTGATACAAATTTACTGC41.702127659574468No Hit
GGACTTAGTAGCTCAAGCCTTCCAGAAGCAGGTGAGCCCTGCCCAGACTT31.276595744680851No Hit
TCAGTTGTAGCTCACCTGAGACCGCGATGGTGAGGTTCCCGAGAGGCATT31.276595744680851No Hit
TATTATAGTAGCTCACAGTTGTTTTAAAAATAGCCCAAACAAGCAGATTT31.276595744680851No Hit
TTTTATAGTAGCTCATGAACCCCAGCCCCTCCAGCCTCTCATACACCCTT31.276595744680851No Hit
CAGGTAAGTAGCTCACCAAGGTTTGGGATGATCAGGCTGTATGGAATGTG31.276595744680851No Hit
TGACCGTCAGTAGCTCACACATCCACATCTGGGCCCTCTCCTTTGAAGCC31.276595744680851No Hit
GAACCGCAGTAGCTCACCTCGAGGCTGCGAACGAGGCTCCTCACCCCACA31.276595744680851No Hit
ACCACCTCAGTAGCTCATCCAGATTCTCTTCCTTCTCCAGAGCTTCTTCC31.276595744680851No Hit
GCTAAGGTAGCTCAGGACTCTTCTTTGGCAGCGCCATTGTCACGTACATG31.276595744680851No Hit
AACTCTTCAGTAGCTCACTTTTTTTTAAAGGAAAATTTGTATTACTTTAA31.276595744680851No Hit
TATGGAGTAGCTCATCTAGAGCCTTGGCCTTTTCTTTAGGAGTTTGTGGC31.276595744680851No Hit
CCAATAGTAGCTCATAAGAGAATACTAAGAAGGTCATATGACTATCAAAT20.851063829787234No Hit
CGATCCCAGTAGCTCACCAAGACTATGACGTCGACGCCCGCCTGGGTGAG20.851063829787234No Hit
CGCTTAAGTAGCTCAACAAAGGTCCCTGCACTCCAGGGCCCAGGGGATTG20.851063829787234No Hit
GGTGGAGTAGCTCATAATATTTTATTCGTCGCCAGATTCTATATGCCAGC20.851063829787234No Hit
CGGGGGGTAGCTCAAGTAGGCTTCATGGTGTGATGTCTGGATAATTGACA20.851063829787234No Hit
TAACGCGTAGCTCACATTGGCAATTTTGGTTAAGTTGTGCTCAGGGGAAC20.851063829787234No Hit
TGGGAACAGTAGCTCAGGCCCACTTGGGCCGGACGTCAGCCTCACTTGCC20.851063829787234No Hit
TTAGGTGTAGCTCAGATTCAGTCAGAACTTTATGTAAGACCTGATGACGT20.851063829787234No Hit
AGCATGGTAGCTCAATTAACACTCATAGGTTACCACTGGCAGATGGTTTA20.851063829787234No Hit
CGTGTTTCAGTAGCTCATTGAATGAGTCAGCAGACATAACTTTTGCCACT20.851063829787234No Hit
TTTTCTTCAGTAGCTCAGGCAGCCATGGTGGTGGTGGATATGGTGGCAGT20.851063829787234No Hit
TTAGGCGTAGCTCACAGGAGGAAGTTGTCCTTCTGGAGTTCAGTGAGGGA20.851063829787234No Hit
CCCGTCTCAGTAGCTCACTCAGTGCCGTGGCCTGTGAGGACCAGAGGAAG20.851063829787234No Hit
GAGCCCTCAGTAGCTCATGTCAATTTTCTTCTCCACGTTCTTCTCGGCCT20.851063829787234No Hit
GCTTACTCAGTAGCTCATAAAACACAAGTCAAACTTAGTAGAGCTGTCCA20.851063829787234No Hit
ATAAGGAGTAGCTCACGTGGAGGCGTTGGCAGAGCAGGCAGACCAAGGAG20.851063829787234No Hit
GCAAAGAGTAGCTCACCTGAAGCCGATCAGCCTGTCAGACTCACCCAGCT20.851063829787234No Hit
TAGGTGTCAGTAGCTCATGACTCAGTCTCTCCTCACCCCCTAGTTGACAG20.851063829787234No Hit
GCGCAGGTAGCTCACGCATCGCGGAAGCCCTCAGCAAGATGAAGCTGCAG20.851063829787234No Hit
TAATGTGTAGCTCACTCTTCACGGACGATCCCTCGGGCAGGGACCTGGAG20.851063829787234No Hit
ACTATTAGTAGCTCAATCCATGGCAAATGCTGGACCCAACACAAATGGTT20.851063829787234No Hit
AACCGCTCAGTAGCTCACCACGGACTTCCAGGAGATCTTTCCCATGGGTG20.851063829787234No Hit
GCGGCCAGTAGCTCACGATGACTGGGTAGTGCAGAGGGCGACAGATGGCT20.851063829787234No Hit
CGAGAAGTAGCTCAAACGCCCCACAAGTGTTACCATGGCAAAACTGGAAG20.851063829787234No Hit
ATAAGAGTAGCTCACCCTGGTGGCCAGGATCACCCAGAATCCCGCGTTTG20.851063829787234No Hit
GTCGCAAGTAGCTCAACAGCCCAGTAAGTCAGGCGCTTCTGCGGCGGCTC20.851063829787234No Hit
TCGCTCAGTAGCTCAAGTAGTGCATGTAATCTGCAACATTCTGGCCCATG20.851063829787234No Hit
TGTGGCCAGTAGCTCACCAGCCAGTGCCCAGGGCTCTCCAGCCAGTGCCC20.851063829787234No Hit
CTCAACCGTAGCTCACGAGGGGTCAGGCCCTGCTTGGGCAGCTGCCTTTT20.851063829787234No Hit
CTTCGTAGTAGCTCAGCAGCTTCTGCCGGTGCACAGGGAAGGTGCTGGCT20.851063829787234No Hit
GAAGACCAGTAGCTCAGCCAGTTCCTGGACCACGACCTCGACTCCACGGT20.851063829787234No Hit
GTATCCAGTAGCTCAATGGGAAAAAGTGGTTCAACGGTTGGTAGAGAGAG20.851063829787234No Hit
TGAGACTCAGTAGCTCATACACAAAATGCCATGTTTGTTTAAGAAGAAGA20.851063829787234No Hit
TAGTCATCAGTAGCTCAGGCGGAATTTTCTACAAAAGGATCCATGGCAGA20.851063829787234No Hit
GAAGGTTCAGTAGCTCATACAAAGCTGTTTACACACAGGAAGGGGCAGGC20.851063829787234No Hit
TTGGACCAGTAGCTCACCTCGCTGAGTGCCCTGCTGCTCCTGCTCACCCC20.851063829787234No Hit
CAAAAGTCAGTAGCTCATGCCTGGTAGAAGACTTCCTCCTCAGCCTATTC20.851063829787234No Hit
GGGGGATCAGTAGCTCACAGCCCCTCGGGCGCCTCGGGCTGGGGCAGAGC20.851063829787234No Hit
ATAGCCAGTAGCTCAGCCATTGACGTGGGGAGCCAGGATGAGACGGACCT20.851063829787234No Hit
GTGGACTCAGTAGCTCAGCAGCTGTGAAGGGCGCAGGAATCAGCGAGGTG20.851063829787234No Hit
GGACTTAGTAGCTCAGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTC20.851063829787234No Hit
TGATTATCAGTAGCTCACAAAATCTACTGTATGGTAATAAGGAGTAAGAA20.851063829787234No Hit
TATTGTAGTAGCTCAAGGAATCATGTAGGAATGATTCCAGTTGCTTAAAC20.851063829787234No Hit
ACACGTGAGCTCACCCGGGTGCCTGGCCCTTCGATTCTGAAACCAGATCT10.425531914893617No Hit
TAAATGTCAGTAGCTCACCTGATCTCTGATCTGTGACCACTCTGTTTTCA10.425531914893617No Hit
AGTCAGTCAGTAGCTCATGTCTAGCATAGTTGGTTAAGCTTATATTTTTG10.425531914893617No Hit
GGTGGTAGTAGCTCACCACAAAATCTAATAGATGTCTTTGTATTCCTCCT10.425531914893617No Hit
AGACCCAGTAGCTACTCGCGAACAGTAGCTCAGAAGATATGCAAGCAGAA10.425531914893617No Hit
ATAAGCAGTAGCTCAGCAGCTTTAAGGAATCTCATGGCAAATAGGCCTGC10.425531914893617No Hit
TCTACGAGTAGCTCAAGGCATTTCTTAGCCCAGAGAACCCTGAAGAACCT10.425531914893617No Hit
ACATTAAGTAGCTCACTGGGTAAAGTGGACAGACCAGAGATCCCAGGAAG10.425531914893617No Hit
GGGTGTAGTAGCTCATAATCTAAGAATCTAAACAGAAACAGAAACAAAAC10.425531914893617No Hit
CCATGTCAGTAGCTCATAACTGTCCCAGGTCAGTTAAGCCCGTGGGAGCC10.425531914893617No Hit
AAAACCAGTAGCTCAGCCTCAGAACTGACCACCAGCGTGGTGCTGACAGG10.425531914893617No Hit
TCTTTATCAGTAGCTCATGGCAAAGGGTAAGATTTTATTAAGACAGACCA10.425531914893617No Hit
GACAATGTAGCTCAAAAGAAGAGCCATGCTGCATGCTCTGGGGTCCCTCC10.425531914893617No Hit
CAAACACAGTAGCTCAGGTTTATCAAGCTCTCAAAGCCACCCAGGCAAAG10.425531914893617No Hit
TTTTGTCAGTAGCTCAGGTGGATATAATAGATTTGGACGTGACGGTGGCA10.425531914893617No Hit
TGGGGTTCAGTAGCTCAATAAAATATCTTTTTCTGGGGGGACTAGGTGGG10.425531914893617No Hit
GATCGGGTAGCTCACCCACCTGGAGGAGGAGGTTGGAGGGCTGCCCTGCA10.425531914893617No Hit
CAGCCTTCAGTAGCTCACGTCTTCGGACGAGGAGTGCTTCTTTGACCTGT10.425531914893617No Hit
GCGACTGTAGCTCACGGGGTCAGAGAGCCAACAGCCTACTCTGACTCAAG10.425531914893617No Hit
TCGTCGCAGAAGCTCACAAAAAAAAAAAAAAAAAACAAAAAAAGGCAAAA10.425531914893617No Hit
GGTCTTAGTAGCTCACATTGATATCAAATACCCTGACCTCGGCCCGGAAG10.425531914893617No Hit
TTACCGTCAGTAGCTCAGTTCACCCTGGGTGCCAGGCCTCTGCCCTCTGT10.425531914893617No Hit
GGGAAGAGTAGCTCACCTCATTTAAGAGGAAAACTGAGCCAGGCTAGGAA10.425531914893617No Hit
GAAAACAGTAGCTCAGCCGGCTCCTGTCCCTCCCACCCCAGGGCCCTGGG10.425531914893617No Hit
TTGTCGTCAGTAGCTCACGTGATCCGCCTGCCTCAGCCTCCCAAAGTGCT10.425531914893617No Hit
GAGGCCAGTAGCTCATGACGATGTCCACCAGCTTGCTGCCCATGGTGCCT10.425531914893617No Hit
TCCGACGTAGCTCACCACCACAACTTCATTCTTGTTGCAGCATTTTCCAC10.425531914893617No Hit
AAGTTACAGTAGCTCATTTCTCTACAGCATCCACAGCTCTGGGGAACAGC10.425531914893617No Hit
ACAGAAAGTAGCTCATGAATTTTTTACATCTGCCTGGGTCCAATGTGCTG10.425531914893617No Hit
CTTCCATCAGTAGCTCAGCAGGAATGCTAGGTGTGGTTGGTTGATGCCGA10.425531914893617No Hit
AACTAAGTAGCTCATTGTGGACGAAGGTCACCGCATGAAGAACCACCACT10.425531914893617No Hit
CGCTCGTCAGTAGCTCACAGGAGGGGAGGCCGGCCCGGCTGAAATACGCT10.425531914893617No Hit
ACCCGCCAGTAGCTCAGCAGATCCTTCGTACCGGGAGGGACCTCTTCAGC10.425531914893617No Hit
ACCCACCAGTAGCTCATATACTAAATAATAAATTATAACATTGATCATAC10.425531914893617No Hit
TATCATTCAGTAGCTCATCAGACATGCATGGCTGTCTGAGGCCCTTCCTG10.425531914893617No Hit
CGGCTTAGTAGCTCACCCTTCTCTGTTCCAGGCCCTGGTCAAATTCCTGA10.425531914893617No Hit
TCATGGCAGTAGCTCACTTCAGGGTAGCAGATGATGTAGGGCCCTCCCCG10.425531914893617No Hit
ATTCAAAGTAGCTCAGCACTAAGGATACATTCTTGCAAGCTTACTTCACA10.425531914893617No Hit
ATGCTCTCAGTAGCTCATTCCTGACTACTGCAGGACATAAGTTCATGGCA10.425531914893617No Hit
AAGCTGTCAGTAGCTCACAGGAGGGTAGCTGATCCTCCGTGAGGTCTGCA10.425531914893617No Hit
AGGAATCAGTAGCTCATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGT10.425531914893617No Hit
TCTGTGAGTAGCTCAAGGAGAGTTGTAAGACTGAGGTCCTGCCTGCCAGG10.425531914893617No Hit
GGAAGGTAGTAGCTCAGGTGCTACATCAGGTGTTGTGATAGACATGGGGA10.425531914893617No Hit
AATATTGTAGCTCAAGTAGGAAAGAGTGACTCATGAAGCTACGGAGTCAA10.425531914893617No Hit
GGACGAAGTAGCTCACACTGCCAAGACTGAGTGGTTGGATGGCAAGCATG10.425531914893617No Hit
TATATTCAGTAGCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.425531914893617No Hit
CGGGGTGTAGCTCACTGAAAGGTGAGGAGGGGGCGGCCAGGGGTGCAGAG10.425531914893617No Hit
CCCGACCAGTAGCTCACAGGCACCGGGGCCTCTGGACTCAGAGGGTCTGG10.425531914893617No Hit
TAGGTACAGTAGCTCAGAATACAAAACCTTGTCTTTGTTCTGTCACATCA10.425531914893617No Hit
TTATCGAGTAGCTCACCTAAATATCTACTGCAATGAAACCAACAGCAATG10.425531914893617No Hit
GCCGGTTCAGTAGCTCAGCAGGAGGACATAGCGCGGCAGCTGCGTGAGTA10.425531914893617No Hit
TAATACTCAGTAGCTCAAGGGACAATTGATTCAGGGGTCTTGACAGAGGA10.425531914893617No Hit
AAATTACAGTAGCTCAGGCACAGGAGATAGTCCATGATTTGCAGGGACAG10.425531914893617No Hit
GACCCCTCAGTAGCTCATCCAGGTGAGGCCTGGGTGCTCCAGCTCCCCAT10.425531914893617No Hit
CGAGCGTCAGTAGCTCAGAAGGATGTGTCCAGCTCATCGGGGAAATCAGT10.425531914893617No Hit
GTGCGCTCAGTAGCTCAGGACCACGTCTACAACCGGCTCGCTCGATACCG10.425531914893617No Hit
GCTAATTCAGTAGCTCACGCCTACTGCACGGGGAGGAACTGAGCATGGTT10.425531914893617No Hit
GCGCATGTAGCTCAGAAGCCACAGCCCGAGGTGTGCATTGGTCCCTTTCA10.425531914893617No Hit
GCCGGACAGTAGCTCATGTTGAGCTTCACAAAGGTTCCATTGAAGATTTG10.425531914893617No Hit
AAATACTCAGTAGCTCAATGTATGAGCAGTGATGTTTATGTCAGGATTCT10.425531914893617No Hit
ACCATGAGTAGCTCAGGACAGCACGCCTGCCCTCACACCCACGGAGGTAA10.425531914893617No Hit
ACGGCCGTAGCTCAGATGAATGACTAAATGGACGTTATTCTGAGTTTGTT10.425531914893617No Hit
TTGAATGTAGCTCAGGCACTCTGTGTCCATCCAGTCTGTGGCCACCTCCC10.425531914893617No Hit
CAGTTAGTAGCTCATCCCAAGGAATGATTTTCTTAAGGAGATAAAGATCA10.425531914893617No Hit
GTAATATCAGTAGCTCAAGCGTCCCTCATGAGTGATCACTTCTGAGTCCT10.425531914893617No Hit
ATCAAGAGTAGCTCACGGGCTCTGCTGGAGGAGCTTTAGGACGCGGGGAT10.425531914893617No Hit
AGGCTTTCAGTAGCTCACCAGTGTATGTACTGTGATAAGGTGTTTCACCG10.425531914893617No Hit
CAATGGCAGTAGCTCATGTATGCAAACTCTCCCTGCCCATTGGCCACCTC10.425531914893617No Hit
TATCAAAGTAGCTCAGGCGATGCCCTGGGCGGCGTCCTCGTTGGCGATGC10.425531914893617No Hit
TTAAGTAGTAGCTCATGATAAATTTCATACTAAAAATATTTCGGTGGGCA10.425531914893617No Hit
NTATCGAGTAGCTCACCTAAATATCTACTGCAATGAAACCAACAGCAATG10.425531914893617No Hit
AACATTGTAGCTCACGTGGACCGTGTCTCCTTAGAACTGTTTGCAGACAA10.425531914893617No Hit
CTACCACAGTAGCTCATTAGTTATCAGTGTCCCATCATTTGATAAGACCA10.425531914893617No Hit
GTATCACAGTAGCTCACTCCAGCACCACATCCCGGGACTCCAGGGAGCAG10.425531914893617No Hit
GTTTTATCAGTAGCTCACCAATGGGAAAGGGAGGCGGGGCAGCCTCAATG10.425531914893617No Hit
CTATAGTCAGTAGCTCACCTGAGGAGTGGGGTGCCAGGTGGAAGGAGAAG10.425531914893617No Hit
ATAGGACAGTAGCTCAAAAGAGGAGAGGCTCCCGACATATGTTCTCTAGG10.425531914893617No Hit
TCTCCAAGTAGCTCAGCCAAAATGAGTGATGTACAAAGTAAGTCACAATA10.425531914893617No Hit
ATAAATAGTAGCTCAACAAAACAAGGCTTTGCTCTATGGATTTTGTGACC10.425531914893617No Hit
CGCTGCGTAGCTCATGCAACCTCACGCCTGATACACTCTCTGGGGACTAC10.425531914893617No Hit
CTAAGTAGTAGCTCACAGAGGTTTCTGGGTTCCTACCCAGACCCACACAT10.425531914893617No Hit
ATACGTTCAGTAGCTCACCGTGGATGAGGTCCGACGGCCGGAAGATGCGG10.425531914893617No Hit
ATGTATCAGTAGCTCACCCAGAAAGTTGCCTGAGCCAGAGAAGAATAAGT10.425531914893617No Hit
AATGAAGTAGCTCACGTGTGTCCCGAGCTCCTGTGGTTTCAGGTGGCCGC10.425531914893617No Hit
TAGTACGTAGCTCAACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCG10.425531914893617No Hit
TACCATGTAGCTCACGATGCCCTGGGCAGCGTCCTCGTTGGCGATGCCGT10.425531914893617No Hit
ACACCCGTAGCTCACCCCGATGCCATCAGCTCCTCACTTATCATCTCTCA10.425531914893617No Hit
CTTTCACAGTAGCTCACAGTAGGAACCACTAGGCCCATAACCATGGTGTA10.425531914893617No Hit
TATGAAAGTAGCTCAGGCAGCCCACCCAGACTCGCCCTGGAGCCGCACCT10.425531914893617No Hit
GTGCGCCAGTAGCTCATGAGTATGAACTCATGAGAGACAGGCATTTGGAC10.425531914893617No Hit
TGTTTTAGTAGCTCACGTGAAATTGCGGCCGGGGCTCACCGCGATGGCCC10.425531914893617No Hit
TCCTAATCAGTAGCTCATTTATGTAGAGCGATAGAGATACTATAGCTTCC10.425531914893617No Hit
CAAATAGTAGCTCAAAAACAAAACAGAGCCATTAAAGGGGCCTATATCAT10.425531914893617No Hit
AGGAACGTAGCTCACCAGGCTCCCAGCACGGCGCACGGCCCTCACAGTGA10.425531914893617No Hit
TCCGAAGTAGCTCACTAAAAACACTAAATCCCGGCACACTCAGGCGGGAG10.425531914893617No Hit
TATGCAAGTAGCTCAGCTGAGGGCAAACATCGAGGAACTGCGCTGCGCCA10.425531914893617No Hit
TCAGTAAGTAGCTCACGCCGCAGGAGGAGCAGCGGAAGGGCCGGCCGCCC10.425531914893617No Hit
TATAGGTCAGTAGCTCACCATGAGCAGGAGCAGGATGTTGGGGGGCTGCG10.425531914893617No Hit
CAAGTTGTAGCTCATCACAATCACGTTACCATATCAAGCTGAAAATGTCA10.425531914893617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)