FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003291851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003291851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262041
Sequences flagged as poor quality0
Sequence length8-619
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG262222.077745493212978No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT228961.8142041344140165No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA226541.7950288461309896No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT206221.6340198139363142No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTGGAAAG34010.26948411343213097No Hit
AAATCTTACCCCGCCTGTTTACCAAAACATCACCTCTAGCATCACCAGTA18450.14619176397597225No Hit
AATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTA16880.1337515976105372No Hit
GGCAGGTCAATTTCACTGGTTAAAAAGTAAGAGACAGCTGAACCCTCGTG16820.13327617723988366No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT16310.1292351040893283No Hit
ATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCA13000.10300774697493979No Hit
AAATCTTACCCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAG12880.10205690623363267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)