FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7515467
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC3673424.887813358770653RNA PCR Primer, Index 15 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2836363.7740302764951266RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1524042.027871321901886No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1001511.332598493214061RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT940921.2519780873231163RNA PCR Primer, Index 1 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC918241.221800321922776RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG760291.0116337414561198RNA PCR Primer, Index 15 (100% over 36bp)
CCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAAT756101.0060585722750164RNA PCR Primer, Index 15 (100% over 41bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAAT694220.9237217061827295RNA PCR Primer, Index 15 (100% over 41bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG692640.9216193750834113RNA PCR Primer, Index 15 (100% over 45bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA634040.8436468419061649RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA605820.8060976117651771RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC596250.7933638721319646RNA PCR Primer, Index 15 (100% over 44bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT550070.7319172581025238RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA529940.7051324954257666No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA522110.6947139811804109RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC515790.686304656783138RNA PCR Primer, Index 1 (100% over 27bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTAT487820.6490880739679916RNA PCR Primer, Index 15 (100% over 48bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC435530.5795115592949847RNA PCR Primer, Index 15 (100% over 44bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG417280.5552283045085555RNA PCR Primer, Index 15 (100% over 36bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA406820.5413103403953474RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA393030.5229615139019305RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG384690.5118643991118582RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG372570.4957376567550626RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA361720.4813007628135417RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG358310.4767634532890638RNA PCR Primer, Index 1 (100% over 29bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTC341020.453757564233866RNA PCR Primer, Index 15 (100% over 38bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA330520.43978637654852315RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT318610.4239390579454344RNA PCR Primer, Index 15 (100% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA310940.41373343798861734RNA PCR Primer, Index 1 (100% over 34bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT307020.40851752791942264RNA PCR Primer, Index 15 (100% over 43bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC300660.4000549799500151No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC297260.3955309763185708No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC291380.3877071112147788RNA PCR Primer, Index 15 (100% over 50bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA262720.34957242178030984RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA262130.34878737409132393RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA248430.33055830063521RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA241960.32194938784243216No Hit
TGAGGTAGTAGGTTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA233030.31006722536337394RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC231620.3081910944456279No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC225730.3003539234488023RNA PCR Primer, Index 15 (100% over 42bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG223730.29769274484207037RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA222270.295750084459156No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC219040.29145228100928394RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC213500.2840808162686364No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC212480.28272361517920314RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC205030.27281072486912655No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT203860.27125393538418835RNA PCR Primer, Index 15 (100% over 43bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG200530.26682307300397967RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC194290.25852019575097596No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA190130.2529849442489735RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT189910.25269221460223296RNA PCR Primer, Index 1 (100% over 35bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG189780.2525192379927954RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA188210.25043021278651084RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA184040.2448816553914747RNA PCR Primer, Index 1 (100% over 34bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG183560.244242972525859RNA PCR Primer, Index 15 (100% over 45bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC172710.22980607858433816RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG171320.2279565594526594RNA PCR Primer, Index 1 (100% over 29bp)
AAACCTGTAGCTGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA166760.2218890722293106RNA PCR Primer, Index 1 (100% over 34bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA159240.2118830406679984RNA PCR Primer, Index 15 (100% over 39bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC155680.20714614274801552RNA PCR Primer, Index 15 (100% over 44bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA152580.20302131590758096RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT149030.19829772388063174RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC146660.1951442272316544RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA146630.19510430955255342RNA PCR Primer, Index 1 (100% over 28bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC145550.19366727310491816RNA PCR Primer, Index 15 (98% over 50bp)
AGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA144740.1925894957691917RNA PCR Primer, Index 15 (100% over 40bp)
TGAGGTAGTAGATTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA144160.19181775397323944RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA144050.1916713891498692RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA142390.18946261090628166RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA139980.18625589068516968RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA136680.18186494598406194RNA PCR Primer, Index 1 (100% over 34bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG136640.1818117224119273RNA PCR Primer, Index 1 (100% over 29bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT134120.17845863736744502RNA PCR Primer, Index 15 (100% over 46bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC132230.17594382358408334RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA131520.17499910517869346No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA129650.1725109031813991No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC129550.1723778442510625RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG128780.17135329048747072RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA126320.1680800408011904RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC123750.16466042629153982No Hit
CCCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA123400.16419472003536173RNA PCR Primer, Index 15 (100% over 40bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA121720.1619593300057069RNA PCR Primer, Index 15 (100% over 39bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA121660.16187949464750495RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC120760.16068196427447556RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC118810.15808731513291188No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA116480.15498704205606917No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC116250.154681006516295RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA113470.15098196825293758RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC113450.15095535646687025RNA PCR Primer, Index 15 (100% over 44bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTA112340.14947840234013401RNA PCR Primer, Index 15 (100% over 47bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG112030.14906591965609056RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA110190.14661763533789718RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC109140.1452205165693629No Hit
TATTGCACTCGTCCCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT105610.140523536328481RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA103880.13822161683365783No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC103800.13811516968938856RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC102350.1361858151995079RNA PCR Primer, Index 1 (100% over 27bp)
AGGATGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA99380.13223396496851095RNA PCR Primer, Index 15 (100% over 40bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT98620.13122271709795277RNA PCR Primer, Index 1 (100% over 30bp)
AAACCGTTACCATTACTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT96250.12806922044897542RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG94280.12544795952134444RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA93970.12503547683730099RNA PCR Primer, Index 1 (100% over 34bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT93720.1247028295114595RNA PCR Primer, Index 1 (100% over 30bp)
GGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC93380.12425042914831508RNA PCR Primer, Index 15 (100% over 44bp)
GCCCGGCTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT90120.11991270801934198RNA PCR Primer, Index 15 (100% over 37bp)
AAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA88200.1173579765568793RNA PCR Primer, Index 15 (100% over 39bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT83900.1116364425524056RNA PCR Primer, Index 15 (100% over 43bp)
ATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA83500.1111042068310592RNA PCR Primer, Index 15 (100% over 40bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA81890.10896195805263997No Hit
CCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC80830.10755153339107204RNA PCR Primer, Index 15 (100% over 42bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG80820.10753822749803839RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT79300.10551573175692208RNA PCR Primer, Index 15 (100% over 43bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA79150.10531614336141719RNA PCR Primer, Index 15 (100% over 40bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA78720.10474398996096981RNA PCR Primer, Index 1 (100% over 32bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT77760.10346662422973849RNA PCR Primer, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA76840.10224248207064178No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA76300.10152396384682416RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph