FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7515467
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3068784.0832858423834475Illumina RNA PCR Primer (100% over 45bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2688793.5776752129974088Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1418801.88784010361565No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC906961.2067912745808078Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT891191.1858078812667263Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA846171.12590475082919Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT707950.9419906973179444Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG706050.9394625776415491Illumina RNA PCR Primer (100% over 36bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT684350.9105887897585074Illumina RNA PCR Primer (100% over 31bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG654800.8712698758440427Illumina RNA PCR Primer (100% over 40bp)
GCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG645400.8587623363924025Illumina RNA PCR Primer (100% over 36bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT582870.7755605872529279Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG573780.7634655304853312Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC568910.7569855605779388Illumina RNA PCR Primer (100% over 39bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT524540.6979473131875903Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT520310.6923189204343523Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG499760.6649753102501814Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA479710.6382969947176935No Hit
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG425370.5659927719727863Illumina RNA PCR Primer (100% over 43bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC402900.5360944303261528Illumina RNA PCR Primer (100% over 39bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT401980.534870288167056Illumina RNA PCR Primer (100% over 31bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT365730.48663642592003925Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG359730.47865289009984346Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT352860.4695117415857192Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG351200.46730296334213167Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT342640.4559131189053188Illumina RNA PCR Primer (100% over 46bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG328390.4369522213323537Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG314710.41874975966230704Illumina RNA PCR Primer (100% over 33bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT307040.4085441397054901Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT291630.3880397585406203Illumina RNA PCR Primer (100% over 38bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC276250.36757529505485154Illumina RNA PCR Primer (100% over 32bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA268760.35760918117264034No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA268670.35748942813533746No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT253120.33679876446799645Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT244880.3258347086082608Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT240690.32025953942715735Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT236500.31468437024605395Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG218840.29118616314861073Illumina RNA PCR Primer (100% over 37bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA213710.2843602400223433No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG213360.28389453376616514No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG210760.28043500157741363Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC205210.27305023094373243Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA200670.2670093555064509No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG193720.2577617598480574No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG191210.25442198069660876Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG189150.25168096673167484Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG188760.2511620369033621No Hit
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT187930.2500576477815683Illumina RNA PCR Primer (100% over 38bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG179570.23893392120542875Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT179370.23866780334475557Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG178050.23691142546431246Illumina RNA PCR Primer (100% over 40bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT177810.23659208403150464Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC173450.23079071466882897Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA173240.23051129091512212No Hit
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT171180.2277702769501882Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG165780.2205850947120119Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA158810.211310887267551Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGACAGCTACAGGTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT152870.2034071868055571Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG151440.20150444410174378Illumina RNA PCR Primer (100% over 34bp)
ATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC147070.19568976884603448Illumina RNA PCR Primer (100% over 39bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT141040.18766631534673758Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT138100.18375438279484163Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT136360.18143915740698482Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT135540.18034807417822474Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAATGATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT133630.1778066486087957Illumina RNA PCR Primer (100% over 35bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT130340.17342900980072162Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTTCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA130050.1730431389027455Illumina RNA PCR Primer (100% over 45bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG128290.17070130172882136Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG126580.16842599402006556Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT126530.16835946455489725Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT122660.16321008395087092Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC121200.1612674235679566No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG120410.16021625801829747Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG120210.15995014015762427Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA118040.1570627613693201No Hit
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT117630.15651721975494004Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGGGGCTCGTCCGGGATGATCGTCGGACTGTAGAACTCTGAACGTGTAG117550.15641077261067077Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC117200.15594506635449268Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC116130.15452133579989108Illumina RNA PCR Primer (100% over 41bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC115550.1537495940039388Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT115330.1534568643571983Illumina RNA PCR Primer (100% over 35bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA114220.15197991023046206No Hit
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG113210.15063601503406243Illumina RNA PCR Primer (100% over 34bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT110020.14639143515632494Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC109470.14565961103947367Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT109040.1450874576390263Illumina RNA PCR Primer (100% over 39bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA108250.14403629208936716No Hit
ACAAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC107900.14357058583318907Illumina RNA PCR Primer (100% over 39bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA107220.14266578510690023No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT105330.14015097132353851Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT102290.13610597984130593Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG102010.13573341483636348Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG99910.13293917729929491No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG98790.131448917279525Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC96780.1287744327797594Illumina RNA PCR Primer (100% over 42bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA94100.1252084534467386No Hit
CGGCCATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT93010.12375811110606966Illumina RNA PCR Primer (100% over 35bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG92730.12338554610112717Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTGT89770.11944700176316388Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT87380.11626689332811921Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG86840.11554837510430156Illumina DpnII expression Sequencing Primer (95% over 24bp)
ACCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC86220.11472340973621466Illumina RNA PCR Primer (100% over 39bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT85560.11384522079599313Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC83040.11049213575151084Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC82870.11026593556993863Illumina RNA PCR Primer (100% over 32bp)
TAATGATCCTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG82540.10982684109982788Illumina RNA PCR Primer (100% over 34bp)
TGGGCCCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT78360.10426497781175807Illumina RNA PCR Primer (100% over 35bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT78180.1040254717371522Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG76710.10206950546120422Illumina RNA PCR Primer (100% over 37bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC76100.10125784598615097No Hit
GGCCGTAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT76040.10117801062794901Illumina RNA PCR Primer (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)