FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190261
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3979654.859002661819935Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT2050762.5039006693437487Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1739272.1235831190239236Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1171521.4303817668325831Illumina RNA PCR Primer (100% over 45bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1082991.3222899734208715Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT953841.164602690927676Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT951731.1620264604510162Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT931351.1371432485484894Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT853931.0426163464143574Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA848861.036426067496506No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC797260.9734244122379005Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG739470.9028650002728851Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT705220.8610470411138302Illumina RNA PCR Primer (100% over 38bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG645550.7881922200037337Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT628940.7679120360144811Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT611320.746398680090903Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG547640.6686478001128413Illumina RNA PCR Primer (100% over 43bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG521210.6363777662274743Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT459040.5604705393393447Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT455310.5559163499185191Illumina RNA PCR Primer (100% over 31bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT416790.5088848816905834Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG416700.5087749950825743Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC409830.5003869840045391Illumina DpnII expression Sequencing Primer (96% over 30bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT401180.4898256600125442Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG398230.486223821194465Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG361240.44106042530268574Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT360510.4401691228155Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGAGCCCGTCTAAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT343290.4191441518164073Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA328920.40159892340427245No Hit
GTGAGCCCGTCTAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC307770.3757755705221116Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT296810.362393823591214Illumina RNA PCR Primer (100% over 38bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA292260.3568384450751936Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT291370.35575178861821377Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT280230.3421502684712978Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG265650.32434863797380814Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG258260.315325726493942Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG255250.31165062993719006Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG253280.3092453341840999Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG250770.30618071878295455Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT246800.30133349840743784Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT246380.3008206942367282Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA240700.2938856283090368No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG238670.2914070748172738Illumina RNA PCR Primer (100% over 40bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT234350.28613251763283243Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC225290.2750705990932401Illumina RNA PCR Primer (100% over 39bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG220700.2694663820847712Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG216420.26424066339277835Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG212390.2593201852785888Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC202750.24755010859849277Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC200570.2448884107600478Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA196220.23957722470627005No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG190890.23306949558750326Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA190190.23221482196965396No Hit
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT189690.2316043408140473Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC184040.2247059037556923Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG181830.2220075770479109Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC181220.22126279003807084Illumina RNA PCR Primer (100% over 39bp)
GTGAGCCCGTCTAAACATGATCGTCGGACTGTAGAACTCTGAACGTGTAG180460.22033485868154873Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGAGCCCGTCTAAACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA170560.20824733180053726Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC169950.20750254479069713Illumina RNA PCR Primer (100% over 32bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT167810.2048896854447007Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG164430.20076283283279986Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT162910.19890697011975567Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT161360.19701447853737505Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG157620.1924480794934374Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT157600.1924236602472131Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG151020.18438972823942973No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT142710.1742435314332474Illumina RNA PCR Primer (100% over 46bp)
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC138090.168602685555442Illumina RNA PCR Primer (100% over 42bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC136790.16701543455086473Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG136130.16620959942546398Illumina RNA PCR Primer (100% over 37bp)
GCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC133780.16334033799411277Illumina RNA PCR Primer (100% over 39bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT132910.16227810078335722Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA128690.15712563983003716No Hit
GCCCGGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG123720.15105745714330715Illumina RNA PCR Primer (100% over 36bp)
CAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGTG122210.1492138040533751Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG120650.14730910284788237Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT120250.14682071792339707Illumina RNA PCR Primer (100% over 35bp)
GCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACG119770.1462346560140147Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTGAGCCCGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT116130.14179035320119837Illumina RNA PCR Primer (100% over 35bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA114180.13940947669433246Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT110100.13442795046458225Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAGATCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG109080.13318256890714472Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTG108750.13277965134444433Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG107410.13114356184741854Illumina RNA PCR Primer (100% over 40bp)
ACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG106120.1295685204659534Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT104720.1278591732302548Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG102300.12490444443711866Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA101130.12347591853299913No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT98310.12003280481537769No Hit
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG96490.11781065340896951Illumina RNA PCR Primer (100% over 34bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG95630.1167606258213261Illumina RNA PCR Primer (100% over 40bp)
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC93810.1145384744149179Illumina RNA PCR Primer (100% over 41bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA93660.11435533006823592Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG92960.11350065645038662Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT92660.11313436775702264Illumina RNA PCR Primer (100% over 31bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG90410.11038720255679277Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG89970.10984997913985892Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA88870.10850692059752431No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC88090.10755456999477794Illumina DpnII expression Sequencing Primer (100% over 26bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG87760.10715165243207755Illumina RNA PCR Primer (100% over 33bp)
AGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG87540.10688304072361064Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT87370.10667547713070438Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT86310.10538125708081829Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT85160.10397715042292303Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG84650.10335445964420427Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT84250.10286607471971894Illumina RNA PCR Primer (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)