FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7643909
Sequences flagged as poor quality0
Sequence length126
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2098772.745676328695174No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC1747602.2862647893898265RNA PCR Primer, Index 16 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1698922.22258009612621RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1279551.6739471911557293No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA928281.2144048287335707RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA808451.0576394878588953No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA779901.0202894880093418No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA765181.001032325214756RNA PCR Primer, Index 16 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC737660.9650298034683563No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG713790.9338023254855597No Hit
CGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC594720.7780312402986482RNA PCR Primer, Index 16 (100% over 42bp)
GGGGATGTAGCTCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA588610.7700379478614934RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC502580.6574908204689511No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA431670.5647241483382389No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG431490.5644886667279791RNA PCR Primer, Index 16 (100% over 45bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA380270.4974810662973618No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC372480.4872899454977813RNA PCR Primer, Index 1 (100% over 26bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC371420.48590322045958423No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC367340.48056563729369356RNA PCR Primer, Index 5 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT354490.4637548667834742RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG346740.45361607523061825RNA PCR Primer, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA346450.45323668819186624No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA344020.45005768645335786RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA302690.3959884922753528No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC295970.3871971788256506No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTAT289140.3782619599474562RNA PCR Primer, Index 16 (100% over 48bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA285540.3735523277422586RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC280240.36661870255127316RNA PCR Primer, Index 1 (100% over 33bp)
GGGGGTGTAGCTCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA276330.3615035186839613RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA264140.3455561807446949No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC253520.33166276573936193RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC251660.32922945576667645RNA PCR Primer, Index 16 (100% over 44bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC243320.3183188078246353RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC235150.3076305591811729No Hit
CCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAT232360.30398059422214474RNA PCR Primer, Index 16 (100% over 41bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA223550.29245507763109163No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAT219090.286620366621319RNA PCR Primer, Index 16 (100% over 41bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA219000.28650262581618907RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT216280.2829442370389286RNA PCR Primer, Index 16 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG209100.2735511372518956No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG207480.2714318027595567No Hit
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC202610.26506071697085876RNA PCR Primer, Index 1 (100% over 31bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA189330.24768740705835196RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT187130.24480929848850896RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG182000.23809807259610236RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG180800.2365281951943698Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC176860.23137376439201462No Hit
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT173760.22731824777087217RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA170910.2235897889417574No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC166580.2179251479838392RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT161780.211645638376909RNA PCR Primer, Index 16 (100% over 43bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG161630.2114494037016924RNA PCR Primer, Index 7 (97% over 36bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC159750.20898992910564476RNA PCR Primer, Index 16 (100% over 44bp)
CGCACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAT158220.20698833541843578RNA PCR Primer, Index 16 (100% over 41bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA156070.2041756384069983RNA PCR Primer, Index 1 (100% over 32bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA155240.20308980653746664RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC153690.2010620482268954No Hit
CGCCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA152760.19984539324055273RNA PCR Primer, Index 16 (100% over 40bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG151510.19821010428041466No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA149450.1955151480741071RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA147830.19339581358176816RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG143980.18835912358454293Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA142710.18669767000104265RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC136010.17793252117470265No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG135750.1775923810709939RNA PCR Primer, Index 7 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA133960.17525064728007622RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA133580.17475351943619422No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG128850.1685655860110318No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA126880.16598837060985422No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG126860.16596220598649197No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC126800.16588371211640537RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC124440.16279628655966472RNA PCR Primer, Index 16 (100% over 50bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA124430.1627832042479836RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAA121600.15908091004223102No Hit
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG120690.1578904196792505RNA PCR Primer, Index 16 (100% over 45bp)
ATCGCTTCTCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC117700.15397880848660025RNA PCR Primer, Index 5 (97% over 35bp)
GCCCGGATAGCTCAGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC115890.15161091007232033RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC115590.1512184407218872RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA113770.14883745999592618RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC111360.14568462288078RNA PCR Primer, Index 16 (100% over 42bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC111200.1454753058938823No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT110720.1448473549331893RNA PCR Primer, Index 16 (100% over 43bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC110340.1443502270893073No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG108210.14156369470123206RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG107830.14106656685735008RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC106720.1396144302607475No Hit
GACGAGGTGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT101900.13330875603045508RNA PCR Primer, Index 16 (100% over 37bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA101090.13224908878428562No Hit
GACCTCGTGGCGCAACGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA99700.1304306474606121RNA PCR Primer, Index 1 (100% over 32bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT98540.128913099305604RNA PCR Primer, Index 16 (100% over 46bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG98290.12858604151357636Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC94780.12399415011350869RNA PCR Primer, Index 1 (100% over 31bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT94270.12332695221777236RNA PCR Primer, Index 16 (100% over 43bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA93940.1228952359322959RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT93820.12273824819212265RNA PCR Primer, Index 16 (100% over 37bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC93790.12269900125707932RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG93740.1226335896986738RNA PCR Primer, Index 1 (100% over 29bp)
AGCTTTGCGCAGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC92320.12077590143995698RNA PCR Primer, Index 2 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA89670.11730908884446427No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG89400.11695586642907443RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC89030.11647182089687357No Hit
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG89010.11644565627351136RNA PCR Primer, Index 16 (100% over 45bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT86970.11377686469056605RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGATAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT86920.11371145313216052RNA PCR Primer, Index 16 (100% over 37bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG86740.11347597152190064RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC86620.11331898378172739RNA PCR Primer, Index 16 (100% over 44bp)
GCCCGGCTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT86420.11305733754810529RNA PCR Primer, Index 16 (100% over 37bp)
GCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT85500.11185376487344367RNA PCR Primer, Index 16 (100% over 43bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC85430.11176218869167595RNA PCR Primer, Index 16 (100% over 44bp)
TAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT83270.10893640936855736RNA PCR Primer, Index 16 (100% over 43bp)
GGGCCAGTGGCGCAATGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC83070.10867476313493529RNA PCR Primer, Index 1 (100% over 31bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT81320.10638535859074198RNA PCR Primer, Index 16 (100% over 46bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG80840.10575740763004897No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA80070.10475006963060392RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG79960.10460616420211177No Hit
CGCGACCTCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC79730.10430527103344636RNA PCR Primer, Index 16 (100% over 38bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA79670.10422677716335975RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA79650.10420061253999754No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC79400.10387355474796992RNA PCR Primer, Index 5 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)