FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7643909
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1966722.572924402946189No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1613912.1113673645251403Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1472921.9269198521332476Illumina RNA PCR Primer (100% over 45bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1178501.5417504316181683No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT819201.0717029729160827Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC743910.9732062482690467No Hit
GGCCGTAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT731550.9570365110312014Illumina RNA PCR Primer (100% over 35bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA724240.9474733411923141No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA709270.9278891206057006No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT640440.8378435693046582No Hit
GGCCGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG561400.734440977777208Illumina RNA PCR Primer (100% over 37bp)
CCACTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG553240.7237658114454266Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG442510.5789053742005562No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG406960.5323977561742297Illumina RNA PCR Primer (100% over 40bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA404990.5298205407730522No Hit
AAAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAAC361390.4727816618434364Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC357600.46782346571629774Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA342430.4479775988960622No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT339560.4442229754435852Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA336550.4402851996275728No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT322940.42248017342958955Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAAC305790.4000440088964953No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT302440.3956614344833252Illumina RNA PCR Primer (100% over 31bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC281960.36886886016042314No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA270370.353706460922023No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT268560.3513385625077431Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA261720.3423902613178676Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTGAGCTACACCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG256020.334933343659638Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG249890.3269138865991209Illumina RNA PCR Primer (100% over 43bp)
AAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAACG243390.31841038400640304Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA240910.31516597070948904No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG235520.3081146047133737Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC233170.3050402614683142Illumina RNA PCR Primer (100% over 39bp)
TCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG217090.2840039042850981Illumina RNA PCR Primer (100% over 36bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG216930.28379458729820045No Hit
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT214820.2810342195334874Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC206320.26991425460454854No Hit
GCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG202960.2655185978796974Illumina RNA PCR Primer (100% over 36bp)
GCCGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT200220.26193404447907476Illumina RNA PCR Primer (100% over 38bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT194180.25403232822368765No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA180990.2367767591163108No Hit
CGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA178830.23395097979319218Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT175880.23009169784726635Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT174740.22860031431562045Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG171530.22440089226598592Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC165350.2163160236470633Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG164790.21558341419292146No Hit
CGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAAC158490.2073415578338256No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC156960.20533996414661662Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC153790.20119287134370648Illumina RNA PCR Primer (100% over 39bp)
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGT151400.1980661988519225Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGTGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG146540.1917081953749057Illumina RNA PCR Primer (100% over 36bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT146400.19152504301137024Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT144160.1885946051948028No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT142990.1870639747281136Illumina RNA PCR Primer (100% over 46bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG141680.1853501918978889Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT141170.18468299400215257Illumina RNA PCR Primer (100% over 31bp)
GCCCTCGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT140960.18440826545684938Illumina RNA PCR Primer (100% over 35bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA140900.18432977158676273No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT138890.18170022693886073Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT137610.18002569104367935Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC137510.17989486792686832Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT137130.17939774008298634Illumina RNA PCR Primer (100% over 38bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT136880.17907068229095874Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT131730.17233329177518988Illumina RNA PCR Primer (100% over 31bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT130580.17082882593186288Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG127190.16639392227196845No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC123710.16184127780694407No Hit
GCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAACT118160.15458059482393105No Hit
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG117010.15307612898060402Illumina RNA PCR Primer (100% over 40bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA116880.15290605892874967No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT115650.15129693459197383Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC115260.15078672443641075No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG114330.14957006945006804Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACT113720.14877204843752065No Hit
AGGCCGAGAAGCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC112830.14760772269790234Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTA109500.14325131290809454Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG104970.1373250257165542Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG104800.1371026264179754Illumina RNA PCR Primer (100% over 37bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA104460.13665782782081784No Hit
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT102740.13440767021166788Illumina RNA PCR Primer (100% over 38bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG102190.13368814306920712Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT100250.13115017460307285Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA99170.12973728494151357No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA96530.12628355465770197No Hit
CGCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTAGAA92840.1214561816473744No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC92160.12056658445305929Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT90840.11883971931115349Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCACCTCGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC90370.11822485066214157Illumina RNA PCR Primer (100% over 32bp)
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT88510.11579154068945614Illumina RNA PCR Primer (100% over 38bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG88100.11525516591053084Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA86950.11375070006720385Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTC86930.11372453544384162Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC85770.1122069872888335Illumina RNA PCR Primer (100% over 41bp)
CCGTTGCGCCACGAGGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG84590.11066327451046316Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG83690.10948586645916375Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG83530.10927654947226609Illumina RNA PCR Primer (100% over 40bp)
GAGCTATCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC81580.10672549869445072Illumina RNA PCR Primer (100% over 32bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG81550.1066862517594074No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG81480.10659467557763967Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC81280.10633302934401757Illumina RNA PCR Primer (100% over 39bp)
GCCACTGCGCAAAGCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT81220.10625453547393093Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC81150.10616295929216321No Hit
GCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT81090.10608446542207658Illumina RNA PCR Primer (100% over 38bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC81040.10601905386367107Illumina RNA PCR Primer (100% over 32bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG80700.10557425526651351Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA79780.10437068259185188Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC78860.10316710991719028Illumina RNA PCR Primer (100% over 32bp)
AAGCCTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT78680.10293162830693038Illumina RNA PCR Primer (100% over 38bp)
GGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC76970.10069455300946151Illumina RNA PCR Primer (100% over 39bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT76500.10007968436044962Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)