FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251338
Sequences flagged as poor quality0
Sequence length126
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4524716.239827739377201Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3388764.673289260547501Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2770093.8201087854407003Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1893152.610759559132397Illumina RNA PCR Primer (100% over 45bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1583702.1840107301576617Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1319861.820160637940198Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1008031.3901296560717484Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA892821.231248633010901No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT855521.1798098502648753Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG842971.1625026995018022Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG674730.9304903453679859Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT558730.7705198681953592No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA518820.7154817497129495No Hit
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC516350.7120754817938427Illumina RNA PCR Primer (100% over 32bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG469760.64782527031563Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG433070.5972277116305984Illumina RNA PCR Primer (100% over 43bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT422390.5824993952840152Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT419600.5786518294968459Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT406000.5598966701041932Illumina RNA PCR Primer (100% over 31bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG398130.5490435006615331Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC368990.5088578135510992Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA364810.5030933601495338No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT360430.4970530955804294Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG352230.4857448377113299Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG319990.4412840775040413Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG292000.4026843046069567Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG282310.38932125353969155Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT282160.3891143951640373Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA259690.35812701049102935Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT256060.3531210378001963Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT252130.3477013483580547Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG252080.34763239556617Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA249860.34457089160648696No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG249540.34412959373842456Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT239360.33009080531068885Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA233900.32256116043687383No Hit
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG231380.3190859397258823Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT228730.3154314417559904Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT218790.30172362672930153Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC204160.2815480398238228Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG200120.2759766542395348Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG199410.27499752459477134Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT198470.27370121210733794Illumina RNA PCR Primer (100% over 44bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG190780.263096272715463Illumina RNA PCR Primer (100% over 36bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG186400.25705600814635865Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG186240.2568353592123274Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT185780.2562009935269877Illumina RNA PCR Primer (100% over 38bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG179120.24701648164793863Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC177570.2448789450995113Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG177460.24472724895736483No Hit
GGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG177090.24421699829741766Illumina RNA PCR Primer (100% over 43bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT174890.24118307545448855Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG171960.23714244185004202Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT171510.23652186672307923Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG166910.230178209869682Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG165050.2276131660115692Illumina DpnII expression Sequencing Primer (96% over 26bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT164950.22747526042779967Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC163450.22540667667125708Illumina RNA PCR Primer (100% over 32bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC163030.22482747321942517Illumina RNA PCR Primer (100% over 39bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT154900.21361574925896437Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT143310.19763249210007863Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT139090.1918128764650055Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC137680.18986840773385547Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG136590.18836523687076784Illumina RNA PCR Primer (100% over 33bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC132880.18324893971291917No Hit
ACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGTA131990.1820215800173706Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG128830.1776637635702542Illumina RNA PCR Primer (100% over 37bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG128590.1773327901692074Illumina RNA PCR Primer (100% over 40bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC127770.17620196438229743Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACG127470.1757882476309889Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGATGTCCAGCCACAATTCTGATCGTCGGACTGTAGAACTCTGAACGT124530.17173382346816546Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC123620.17047888265586295Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC122740.1692653135186913Illumina DpnII expression Sequencing Primer (100% over 26bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT121780.16794141991450404Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT120800.16658994519356288Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT119000.1641076446857118Illumina RNA PCR Primer (100% over 46bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG115780.15966708488833373Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT115500.15928094925377909Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA113600.15666074316215847No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG113260.15619186417734218Illumina DpnII expression Sequencing Primer (96% over 26bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT113110.1559850058016879Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA112350.15493692336503967No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT109940.15161339879619457Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC109500.15100661422760875Illumina RNA PCR Primer (100% over 39bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC105070.14489739686661965Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT101140.13947770742447807Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT98680.13608523006374823Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG98140.1353405399113929Illumina RNA PCR Primer (100% over 36bp)
CAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTGT96570.13317542224621168Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG94470.13027940498705204Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT94280.13001738437788998Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA93270.12862453798181797No Hit
ACACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG88740.12237741503705937Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA87880.12119142701664162Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC87310.12040536518915543Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC86990.11996406732109302No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT86400.11915042437685294Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC85590.11803338914831993Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT85470.11786790244779655Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT83430.11505462853889863Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA83230.11477881737135961Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT81190.1119655434624617Illumina RNA PCR Primer (100% over 39bp)
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC78690.10851790386822405Illumina RNA PCR Primer (100% over 42bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC78540.10831104549256979Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG76260.10516679818262506Illumina RNA PCR Primer (100% over 34bp)
GTTCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA75720.10442210803026973Illumina RNA PCR Primer (100% over 45bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT73540.10141576630409449Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA72870.10049179889283881Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)