FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003293403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4157820
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA45932111.047159328686668No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1268863.0517434617179195No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1079512.5963365417454343No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG1062072.554391483998826No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT829401.9947953494860289Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG759191.8259328205646228No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA742821.786561226796735No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG711951.7123155884574126No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA610771.468966910544468Illumina RNA PCR Primer (100% over 45bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG556061.3373835327166639Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT436311.0493720266870619Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT430161.0345806215757296Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA404940.973923835086656No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT369170.8878931747887114Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA363360.8739195058949162No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT334970.8056385317305703Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT285110.6857199205352804Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG279580.6724196814676923No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT269710.6486812800938954Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC259730.62467831700266No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA247770.5959132429975323No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT242740.583815557191028Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG236830.569601377645016Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT230680.5548099725336835No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC218530.525587928289344Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA198260.47683641908500124No Hit
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG190710.4586778648426339Illumina RNA PCR Primer (100% over 43bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC163400.39299440572222943Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG162390.3905652481348399No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA157160.37798654102390195No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG150160.3611507953687269Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA149840.36038116128163317No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG149650.35992419104242124No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA147610.35501777373719884No Hit
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG147580.3549456205415338Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA139480.3354642577119741No Hit
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT132900.31963865679610953Illumina RNA PCR Primer (100% over 38bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT130740.3144436267082269Illumina RNA PCR Primer (100% over 31bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT122300.29414452766113014Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA120880.29072927639965174Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT118450.2848848675507838Illumina RNA PCR Primer (100% over 38bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA110140.26489843235156885No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT109250.2627578875468395Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC109160.2625414279598443No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT103450.24880826971826583Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG103400.24868801439215743Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT102650.2468841845005315Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA100740.2422904310431909No Hit
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT96940.233151026258953Illumina RNA PCR Primer (100% over 38bp)
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA94860.22814840469284384No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA94220.2266091365186564No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT89870.2161469231472262Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC89120.2143430932556003Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC86290.20753664179786524No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT82990.19959979027471125Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG81760.19664150925244478Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA79980.19236041964298597No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT79310.19074899827313352Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG78230.18815148322919223Illumina RNA PCR Primer (100% over 34bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG78190.1880552789683055Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC78080.18779071725086705Illumina RNA PCR Primer (100% over 39bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA75740.18216276798899422No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG74370.17886777205362425Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC72420.17417781433539692Illumina RNA PCR Primer (100% over 39bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT69500.16715490329066673No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG66140.1590737453761827Illumina RNA PCR Primer (100% over 40bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC65840.15835221341953234Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT65350.1571737112236701Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAG61340.14752923406977694No Hit
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT61130.1470241617001217Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC59580.1432962465907615No Hit
ACAAATTCGGTTCTACAGGGTAGATCGTCGGACTGTAGAACTCTGAACGT59440.142959531677658Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA58920.1417088762861307No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC58680.1411316507208104No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA57300.13781260372021875No Hit
GCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA56510.13591256956770617No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA55050.13240111404534108No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG53870.12956308834918298Illumina DpnII expression Sequencing Primer (95% over 24bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG53520.1287213010664242Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG51640.12419970080474864Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA51530.12393513908731017Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA50490.12143382830425559Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT50290.12095280699982201Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT49890.11999076439095488Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC48980.11780211745578212Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC48600.11688817697735833Illumina RNA PCR Primer (100% over 32bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA46950.11291975121578135No Hit
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG46210.11113997238937712Illumina RNA PCR Primer (100% over 37bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT46050.11075515534583026Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT46050.11075515534583026Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG45810.11017792978050998Illumina RNA PCR Primer (100% over 36bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC45600.10967285741085474Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT44090.10604114656238126Illumina RNA PCR Primer (100% over 46bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG43260.10404490814898192Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG42830.10301071234444974Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAGATCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG42610.10248158890957282Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA41810.10055750369183851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)