FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003293404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469368
Sequences flagged as poor quality0
Sequence length126
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT2115943.8687102422071433RNA PCR Primer, Index 7 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1897623.4695416362548652RNA PCR Primer, Index 7 (100% over 50bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1552992.839432270785217RNA PCR Primer, Index 7 (100% over 44bp)
CGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT1370522.505810543375395RNA PCR Primer, Index 7 (100% over 43bp)
CCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT1315302.4048482384070704RNA PCR Primer, Index 7 (100% over 41bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1157612.116533391060905RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT1095292.002589695920991RNA PCR Primer, Index 7 (100% over 41bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1068581.9537540717684383RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1017311.8600138078110668RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG986031.802822556463562RNA PCR Primer, Index 7 (100% over 45bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA885831.6196204022110048RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA623851.1406253885275228RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC613921.122469725935428RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA573581.0487134893830512RNA PCR Primer, Index 7 (100% over 40bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC506370.9258290903080575RNA PCR Primer, Index 7 (100% over 39bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA428740.7838931298826483RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA416820.762099021312883RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT413930.7568150470036026RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA364420.666292705116935RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG347150.6347168447981558RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT331880.6067977141051764RNA PCR Primer, Index 7 (100% over 41bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG326410.5967965585786146RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTAT308640.5643065158533856RNA PCR Primer, Index 7 (100% over 48bp)
CTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC290510.5311582617955127RNA PCR Primer, Index 7 (100% over 42bp)
CCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC282450.5164216414035406RNA PCR Primer, Index 7 (100% over 42bp)
CCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC274300.5015204681784075RNA PCR Primer, Index 7 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC268460.4908428176710728No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA268440.4908062503748148RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG258680.47296140980091306RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA250130.45732889065062RNA PCR Primer, Index 7 (100% over 35bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA245810.4494303546588929RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA239340.4376008343194314RNA PCR Primer, Index 7 (100% over 40bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC238710.4364489644873046RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT232810.4256616120911959RNA PCR Primer, Index 7 (100% over 43bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG198890.36364347763763566RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC195070.3566591240523585RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC188850.34528669491612196RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC187020.34194078730851535RNA PCR Primer, Index 7 (100% over 42bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG184240.336857933128654RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA165350.3023201218129773RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC165030.3017350450728494RNA PCR Primer, Index 7 (100% over 42bp)
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA163650.2992119016310477RNA PCR Primer, Index 7 (100% over 40bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA157580.28811372721674605RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT154460.28240922900049875RNA PCR Primer, Index 1 (100% over 30bp)
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT151870.2776737641350884RNA PCR Primer, Index 7 (100% over 41bp)
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC147340.2693912715326524RNA PCR Primer, Index 7 (100% over 39bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG141570.2588416065622207RNA PCR Primer, Index 7 (100% over 36bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC140640.2571412272862239RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT138510.25324681023474743RNA PCR Primer, Index 7 (100% over 38bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG137880.25209494040262054RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA137600.2515829982550086RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA137090.25065053220042977RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG136870.25024829194159176RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC134790.24644529313076025RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA126800.2318366582756911RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA120520.22035452725068053RNA PCR Primer, Index 7 (100% over 40bp)
CGGCTTAGCCTGTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA119240.21801422029016881RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC112830.20629440183948128RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC111580.20400894582335655RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA108980.19925519730981714RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT106840.19534249661021164RNA PCR Primer, Index 7 (100% over 41bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT105440.19278278587215195RNA PCR Primer, Index 7 (100% over 43bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA101260.1851402209542309RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC97380.1780461654801798RNA PCR Primer, Index 2 (100% over 34bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG97090.17751593968443885RNA PCR Primer, Index 1 (100% over 29bp)
ATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA95430.17448085409502523RNA PCR Primer, Index 7 (100% over 40bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG92500.1691237451932289RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA89280.16323641049569165RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA81950.1498344964171363RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG80140.14652515610578773RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC79440.14524530073675787RNA PCR Primer, Index 2 (100% over 34bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG76280.1394676679279946RNA PCR Primer, Index 1 (100% over 29bp)
CCCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA74850.13685310624554792RNA PCR Primer, Index 7 (100% over 40bp)
GCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT73350.13411055902619828RNA PCR Primer, Index 7 (100% over 43bp)
TATTGCACTCGTCCCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT72990.13345234769355435RNA PCR Primer, Index 1 (100% over 30bp)
CCCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA70180.128314642569306RNA PCR Primer, Index 7 (100% over 40bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA68510.12526127333176337RNA PCR Primer, Index 1 (100% over 28bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT67750.12387171607395955RNA PCR Primer, Index 7 (100% over 46bp)
CGGTGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT67710.12379858148144356RNA PCR Primer, Index 7 (100% over 41bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC67350.12314037014879964RNA PCR Primer, Index 7 (100% over 44bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA66120.12089148142893293RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG65080.11898998202351715RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA65020.11888028013474317No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA64690.11827691974648626RNA PCR Primer, Index 7 (100% over 40bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC64650.11820378515397026RNA PCR Primer, Index 1 (100% over 33bp)
CCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC64630.11816721785771227RNA PCR Primer, Index 7 (100% over 42bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG64400.11774669395074533RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA64060.11712504991435939RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT63800.11664967506300546RNA PCR Primer, Index 7 (100% over 41bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA63410.11593661278597453RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCA63160.1154795215827496RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA61740.11288324354843192RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA61660.11273697436339994RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC59930.10957390323708333RNA PCR Primer, Index 7 (100% over 44bp)
CGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC59780.10929964851514835RNA PCR Primer, Index 7 (100% over 42bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA59320.10845860070121448RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT58330.10664851953644369RNA PCR Primer, Index 1 (100% over 30bp)
CGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT58280.1065571012957987RNA PCR Primer, Index 7 (100% over 43bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC56490.10328432828070812RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT56440.10319291004006315RNA PCR Primer, Index 7 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph