FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469368
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT1900703.4751729998785965Illumina RNA PCR Primer (100% over 38bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1513692.7675775336382555Illumina RNA PCR Primer (100% over 45bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1402422.564135380906898Illumina RNA PCR Primer (100% over 39bp)
AGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT1223792.237534574378612Illumina RNA PCR Primer (100% over 38bp)
TCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1215522.222413997375931Illumina RNA PCR Primer (100% over 36bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1088121.9894803202125Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1011361.8491350371743134Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG991411.8126591591569627Illumina RNA PCR Primer (100% over 36bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT921171.684234814698883Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG911031.6656951954960795Illumina RNA PCR Primer (100% over 40bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT813481.4873382079977064Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG591811.082044579922214Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT559941.0237745933350983Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT517700.9465444636382119Illumina RNA PCR Primer (100% over 35bp)
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG475030.8685281370717788Illumina RNA PCR Primer (100% over 34bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT407640.745314632330463Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT383430.7010499202101595Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT351000.6417560493278199Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT348500.6371851372955705Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG317820.5810909048358055Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG302110.5523672936251501Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG276860.5062010820994308Illumina RNA PCR Primer (100% over 36bp)
TCCGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG263060.480969647681414Illumina RNA PCR Primer (100% over 37bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG261710.47850135518399933Illumina RNA PCR Primer (100% over 43bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT259390.47425954881807186Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG256310.4686281851943405Illumina RNA PCR Primer (100% over 37bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC241220.44103816016768294Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA240510.43974002115052413No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG237200.43368813361982594Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG227360.4156970238608922Illumina RNA PCR Primer (100% over 37bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT227120.41525821630579624Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT221020.4041051909471076Illumina RNA PCR Primer (100% over 35bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC218180.3989126348784722Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG195490.35742703727377645Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT186650.34126429232774247Illumina RNA PCR Primer (100% over 38bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG186550.34108145584645244Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG167750.3067081973639368Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG166430.30429475581090903Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG156710.28652304982952326Illumina RNA PCR Primer (100% over 37bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG156120.2854443145899124Illumina RNA PCR Primer (100% over 37bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT150360.2749129332676097Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT142760.2610173606895715Illumina RNA PCR Primer (100% over 35bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG140740.2573240637675139Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG135350.24746917742598412Illumina RNA PCR Primer (100% over 36bp)
GCCCGGCGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG132540.24233147230173577Illumina RNA PCR Primer (100% over 34bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG131730.24085049680328696Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG130430.23847362254651727Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG129090.23602361369723157Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT124860.22828963053866552Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT123800.22635156383699176Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGGCGGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG122980.22485230469041395Illumina RNA PCR Primer (100% over 33bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT122510.22399297322835107Illumina RNA PCR Primer (100% over 31bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT121080.22137841154590437Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCGTAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT114010.208451872318703Illumina RNA PCR Primer (100% over 35bp)
GAGCCACAGGCTAAGCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG112060.20488656093354843Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT110260.20159550427032885Illumina RNA PCR Primer (100% over 39bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT103940.19004023865280228Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC103180.18865068139499847Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG102940.1882118738399025Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA101130.18490253352855393Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT100640.18400663477023305Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT97870.17894206423850068Illumina RNA PCR Primer (100% over 38bp)
GCCGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG93480.1709155427098707Illumina RNA PCR Primer (100% over 36bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG89430.1635106652176266Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGGCCCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT88040.16096923812769592Illumina RNA PCR Primer (100% over 35bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT84450.15440540844938574Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG83670.15297928389532392Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT82850.1514800247487461Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG77900.14242961892489223Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT74470.136158327616646Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT73930.13517101061768014Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA72030.1316971174731706Illumina RNA PCR Primer (100% over 45bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT69960.12791240231046805Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT69140.12641314316389024Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCCGGCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT67570.1235426104076376Illumina RNA PCR Primer (100% over 35bp)
GCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT67220.12290268272312267Illumina RNA PCR Primer (100% over 38bp)
ACAAGATCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG64110.11721646815500439Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT62470.11421794986184876Illumina RNA PCR Primer (100% over 35bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT61680.11277354165965794Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGTG61440.11233473410456199Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT61090.11169480642004706Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC60890.11132913345746712Illumina RNA PCR Primer (100% over 39bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT60560.11072577306921018Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT60510.11063435482856519Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT59700.10915337933011639Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCACCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG59430.10865972083063345Illumina RNA PCR Primer (100% over 36bp)
ACCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG59170.1081843459792795Illumina RNA PCR Primer (100% over 37bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT58980.10783695666482854Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGGCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT58820.10754441829476458Illumina RNA PCR Primer (100% over 35bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT58680.10728844722095862Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG58430.10683135601773368Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCGGCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG57590.10529552957489786Illumina RNA PCR Primer (100% over 36bp)
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC57420.10498470755670491Illumina RNA PCR Primer (100% over 41bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT57020.104253361631545Illumina RNA PCR Primer (100% over 46bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA56810.10386940502083604No Hit
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT54780.10015782445064951Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)