FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8080159
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA100361712.420758056864969No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3955354.895138820906865Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA2436763.01573273496227No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1738482.1515418199072567No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1583761.9600604394047194Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1574911.9491076846383841Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1319721.6332846915512431Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1019491.2617202210996095No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT987331.2219190241182134Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG915271.13273761073266No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT866151.0719467277809756Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT770040.953001048617979Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA762880.9441398368522205No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA719120.8899824867307684No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA635420.7863954162288143No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT594020.7351588007117187No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC586510.7258644291529411Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA563210.6970283629319671No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA474970.587822591114853No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT474210.5868820155642978Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC467460.5785282195560756No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT466600.5774638840646577Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG446840.5530089197502178No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT424950.5259178686954056Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA406080.5025643678546424No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG399890.49490362751525063No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA353190.43710773513243983No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG334290.41371710630941794Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA317650.39312345214988964No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC316230.3913660609896414Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG290150.3590894684126884Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT289110.3578023650277179No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT276800.342567516307538Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA262770.32520399660452226No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG259090.3206496307807804Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG250510.31003102785477366Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT239000.29578625866149416Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA227530.2815909934445597No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT226910.28082368181121187Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG222340.2751678525137934Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG222270.2750812205551896Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG220630.27305155752504373No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT218900.27091051054812165Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC217270.2688932235120621Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC216610.26807640790236925No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT212920.2635096660845412Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT212870.26344778611410985Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG209820.2596731079178021Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG209290.2590171802312306Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT205020.25373263075639974Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA203870.2523093914364804No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA196750.24349768364706684No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA185860.23002022608713518No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA184720.2286093627613021No Hit
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT184400.22821333095054194Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT183360.2269262275655714Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA175200.21682741639118736No Hit
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG174220.21561456897073436Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC172660.2136839138932786No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA167590.2074092848915473Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG162620.20125841583067855Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC161300.19962478461129293Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA156690.19391945133752936No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG154380.19106059670360448No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA148170.18337510437604013No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA141470.1750831883382493No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG140860.17432825269898775Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG139010.17203869379303058Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT136760.1692540951236232Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC135020.16710067215261482No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC133080.16469972929988136Illumina DpnII expression Sequencing Primer (100% over 26bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT130640.16167998674283512Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA129190.15988546760032815No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA124120.1536108385985969No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC121710.15062822402380943Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG121160.1499475443490654Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT117330.14520753861402974Illumina RNA PCR Primer (100% over 31bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG114990.1423115559978461No Hit
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT112280.13895766160047096Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA111130.13753442228055165No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA109970.13609880696654608No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCGATCG109240.13519535939824945No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG108770.1346136876761955Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA105990.13117316132021659No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT105380.13041822568095504Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG102740.12715096324218372No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT102730.12713858724809748Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAAC98900.12239858151306181No Hit
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC95650.11837638343502893Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG95410.11807935957695882No Hit
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG94190.11656948829843572Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC93940.11626008844627933Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG93120.11524525693120642No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC92870.11493585707905005No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT91180.11284431407847297Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGTG89870.11122305885317356Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT89100.11027010730853193Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA89040.1101958513440144No Hit
GGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA88710.10978744353916797No Hit
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT86890.10753501261546958Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG84760.10489892587509726Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT83960.10390884634819686Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT83760.10366132646647178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)