FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10346933
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA7632917.376978279457304No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA4361734.215481051244847No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3031672.930018006301964Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2825972.7312151339918795Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2246732.1713970700303173Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2141332.0695311354582078Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1391951.3452778712300544No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1299381.2558117463406788No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1031000.9964305364691161No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA974530.9418539774056718No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC971750.9391671908960848No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA877770.8483383433525664No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG848740.8202817201966998No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT780890.7547067329033638Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA763580.7379771377663313No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT745870.720860954642308Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA691650.6684589530056878No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC682230.6593548059120514No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG598370.5783066344394034Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA543150.5249381628353059No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT478230.4621949325466783Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA475550.4596047930338391No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT473900.45801011758750154No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT466820.45116751021776214Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG439420.4246862331088836Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA437870.42318820465929374No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG420390.4062943096277902Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC388100.37508699437794757No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC387490.37449744769778637Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT385820.37288344285209923Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG381630.3688339336883693Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT378190.3655092770002473No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT367960.35562228923295436Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG362960.35078993939556774Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG360800.34870236426581674Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA349080.33737533624698257No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC311170.3007364597799174No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT294680.2847993700162164Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC288350.278681615122085Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA280720.27130744927023304No Hit
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT275060.2658372292543114Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT273560.2643875243030954Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA257410.2487790343283367Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC251450.24301887332217187Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG231290.22353483877782915No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT226800.21919538862385599Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT220520.21312595722809843Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT207700.20073581224503917Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT206430.199508395386343Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT195630.18907051973758796Illumina RNA PCR Primer (100% over 31bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC191750.18532061626377594Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT189860.1834939880252438Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT189840.18347465862589427Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT188220.181908977278581Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG186520.18026597833386956No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA186120.17987939034687864No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA182550.1764290925629846No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG180550.17449615262802998Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA178500.17251488919470145No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT177500.17154841922722416Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC176960.1710265254447864No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG174950.16908392081015697Illumina RNA PCR Primer (100% over 40bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG173100.16729595137032394Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG167920.16228963693879142Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA166560.16097523778302228No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG160840.15544702956905201Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG157720.15243164327052278Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC157350.15207404938255617Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC152740.14761862283248572Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG152140.14703874085199933Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG150850.1457919945939536No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT147600.14265096719965228Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG146850.14192611472404432Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT145710.14082433896112018No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT144530.13968390439949693Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC143120.1383211817453539Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG142720.13793459375836298Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA142160.1373933705765757No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT136760.13217443275219817Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG135590.1310436628902497Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA135580.13103399819057493No Hit
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT134210.129709934335131Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA129910.12555411347497852No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG127330.12306062095888705Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG126240.12200716869433677Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG125770.12155292780962243Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT123520.11937837038279846Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA120340.11630499588622058No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA118760.1147779733376064No Hit
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT116550.11264207470948154Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA116360.11245844541566084No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT114470.11063181717712871Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC110280.10658230801339875Illumina RNA PCR Primer (100% over 39bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA109750.10607007893063579Illumina RNA PCR Primer (100% over 45bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC107480.10387619210446225Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT107400.10379887450706407Illumina RNA PCR Primer (100% over 31bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG104650.10114108209650144Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT104160.10066751181243756Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGTATTCTCGCACGGACTACAACCACGACCAAGATCGTCGGACTGTAGA104060.10057086481568983No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG103870.10038723552186914Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG103800.10031958262414573Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC103670.10019394152837367Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)