FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6737203
Sequences flagged as poor quality0
Sequence length126
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC82004612.171905759704732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1807582.6829828342711357No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1499162.225196420532378RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1175401.7446409140410346RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1168461.7343399033693954No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC1040301.5441125939058093No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA992631.473356228096437No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC928071.377530111531447No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT912281.3540930858102391No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC868161.2886059689755525No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA719121.0673865697678993RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC640810.9511513902727883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA614840.9126042365058616No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC590330.8762241541482422No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT542300.8049334419639722RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC532440.7902982884737182No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA530760.7878046720575288RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA509190.755788418428241RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA446710.6630496364737711No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC433880.644006125390611RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA429510.637519754117547No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA411510.6108024353726613RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC362010.5373298088242258No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG343110.5092766241420957No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC341970.507584527288253RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT339980.5046307792714573RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC335030.49728351661661374No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA331670.49229628378423507RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC329860.48960970895488826RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA324810.482114016751462No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA324330.48140155491826503No Hit
AGGCAGTGTAGTTAGCTGATTGCTGGAATTCTCGGGTGCCAAGGAACTCC316920.4704029253682871RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC311550.4624322586093962No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG308940.45855824739138784No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC306690.4552185825482771No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC302080.4483759803586147No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC294270.43678363261430597No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA287620.4269130676335565RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG279980.4155730501218384RNA PCR Primer, Index 2 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT268950.3992012709131668No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC268440.398444280215395No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT266380.3953866315145914No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC262250.389256491158126No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG257390.3820428150970069No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG241580.3585761034660823RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA237000.35177803014099474No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC235940.3502046769260181No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC221540.3288308219301096RNA PCR Primer, Index 1 (100% over 27bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA215730.32020706515745484No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG215630.3200586356088721No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA204060.3028853368378539RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA203590.30218771795951527RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA182950.2715518591320464RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA182540.27094329798285727No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT172050.25537303833653224RNA PCR Primer, Index 12 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC171140.2540223294444297RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA169970.2522857037260121No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA163890.2432611871721841RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA163840.24318697239789272RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG162780.24161361918291613No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA159930.23738337704830922RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG159810.23720526159000999No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG151710.22518246815481144RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC151290.2245590640507641RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA146670.21770161890624345RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG144160.2139760372368177RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC141590.21016139783824236RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA141550.21010202601880928RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC138890.20615380002650951No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG138400.2054264952384543RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC137240.203704712474895RNA PCR Primer, Index 1 (100% over 27bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC133260.19779721644130363RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC131670.1954371866188387No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAA128950.1913999028973893No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT126510.18777822191197147No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC125220.18586348073525466No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT125130.18572989414153027RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG120020.17814514420895436RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT116720.17324696910572532No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC116060.17226733408507952No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG115220.17102052587698485Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCA114780.170367435863221RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA111720.16582549167659041No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT108860.16158040658712525No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA107850.16008126814644No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA104420.15499013463005346No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTG104310.15482686212661248No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC104120.15454484598430537No Hit
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA102920.15276369140131296RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTGGAATTCTCGGGTGCC102700.15243714639443104No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG101470.15061146294686384RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA100710.14948339837763536RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG100540.14923106814504478RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC96750.1436055882537605No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA96020.1425220525491068RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG95710.14206192094850043RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT94240.13988000658433478RNA PCR Primer, Index 12 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA93130.13823243859506681No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC92920.13792073654304315RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA92840.137801992904177No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG92000.13655518469608233RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG89770.13324520576268817No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTTGGAATTCTCGGGTGC88970.13205776937402658No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG88820.13183512505115252RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC88620.13153826595398715RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCC87110.1292969797703884RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGATGGAATTCTCGGGTG85960.12759003996168736No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA85230.12650650425703366RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC83950.12460660603517512RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA79140.11746714474834734No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA79100.11740777292891427RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA78400.11636876608883538RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG77290.11472119809956742RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA76410.1134150180720397RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA75280.11173776417305521RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCC73380.10891760274998392No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG72330.10735909248986561RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC72130.1070622333927002RNA PCR Primer, Index 2 (100% over 34bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC70110.1040639565113297RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG69970.10385615514331394No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC69270.10281714830323503RNA PCR Primer, Index 1 (100% over 26bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA69270.10281714830323503No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG68810.10213437237975463No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC68200.10122895213340016No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG67760.10057586211963629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)