FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6737203
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA77635311.523372533082348No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1668452.4764728033280283No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1411892.0956619534842575Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1084001.6089763066364484No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1042791.5478084896655186Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG983501.4598046103108366No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA936421.3899239788381024No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG862421.2800861128869059No Hit
GCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGG810101.202427773068438No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA789681.172118459247851No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT692881.0284386562197994Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA583840.866591076445225No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC569370.8451133207653087No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG547390.8124885059868316No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT525760.780383194628394Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT501030.7436765672638928Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG491940.7301843212977255No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT482120.7156085396269045Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG419570.6227658569884268Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC416240.617823153020623No Hit
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT401390.5957813650560922Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA392990.5833132829751456No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA351510.5217447062230424No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT320760.4761026200338627Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA317570.47136771743407463Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC313310.4650446186644517Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA311100.4617643256407741No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTG306620.4551146818642692No Hit
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG303830.4509734974588119Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA302140.44846503808776433No Hit
GCAATCAGCTAACTACACTGCCTGATCGTCGGACTGTAGAACTCTGAACG299820.44502147256064567Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA297410.4414443204398027No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA290340.4309503513550059No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG289670.42995587337950186No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA281860.4183635256351931No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT278600.4135247223513972Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG278590.4135098793965388No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC270970.4021995477945373Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA269770.4004183932115449No Hit
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT257720.3825326326073298No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA242320.3596744821255942No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGT240200.3565277756956411No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT235560.3496406446414039No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA231350.34339176064607224No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG228610.3393247910149063Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC218210.3238881179623057No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA211260.3135722643358082No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA200760.2979871617346249No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG197980.2938608202840259Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT194000.28795332425043446Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA190980.283470751883237No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT189310.280991978421906Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCGATCG177530.2635069775988641No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT169940.2522411748614373Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG164140.2436322610436408Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC158370.23506787609041913No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG156930.23293049059082827No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT152840.22685972205379593No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT152040.22567228566513434Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT151440.22478170837363814No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT150860.22392081699185848Illumina RNA PCR Primer (100% over 31bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC147770.2193343439406531Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG144830.21497051521232177Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT144760.21486661452831388Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT140660.20878100303642327Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT139760.207445137099179Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG135190.20066190672894968Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG135110.20054316309008352Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG133840.19865810782308327Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG130380.1935224454421219No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT130270.19335917293868093Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA127340.18901018716520787No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC123350.18308784817675822No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA122060.1811731070000414No Hit
GAGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGT116060.17226733408507952No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC115080.1708127245089691Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG114300.16965497403002402Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG112700.16728010125270085Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT111020.16478648483651154Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC109890.16310923093752705Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT109210.16209991000716467Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG107840.16006642519158173No Hit
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT106600.15822589878915627Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA104630.15530183668207712No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA102310.1518582711549585No Hit
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT102100.15154656910293485No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATC101100.1500622736171079No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA100970.14986931520395036No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG100070.14853344926670609Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT98230.14580234557278443Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTTAACAATAACCCACAACACTCGGACCAACTGATCGTCGGACTGTAGA96730.14357590234404397No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA96360.143026713014288No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT95830.14224003640679966Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA95410.14161663230275234No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA95100.14115650070214597No Hit
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG93180.13830665336935818Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT90610.13449201397078284Illumina RNA PCR Primer (100% over 31bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG90290.1340170394153182Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT89680.13311161916896372Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGTG88850.13187965391572734Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAAC86580.1285103031629001No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA85840.12741192450338812Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA85540.12696663585764004No Hit
AACTTAACAATAACCCACAACACTCGGACCAACTGATCGTCGGACTGTAG84560.1255120262815296No Hit
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG84430.1253190678683721Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG83930.1245769201254586Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG83110.12335979782708048Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT81760.12135599892121404Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA81570.12107398277890692No Hit
TCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTA80290.11917408455704838No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG76160.11304394420058295Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT74680.11084718688155902Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT74400.11043158414552746Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG73660.10933320548601548Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA73500.10909571820828318No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT73490.10908087525342491Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)