FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7561679
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA4452585.888348341684433No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA4159045.500154132435402No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA3176094.200244416616997No Hit
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG2423083.2044206055295392Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1992442.634917456824073Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1973662.6100817027541106No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1229491.6259484170116185No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA1119151.4800284434184523No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGT884711.169991479405566No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC854781.1304103228925746No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG809241.0701856029593428No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG712900.9427800360211005No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA661860.8752817991877202No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT623760.8248961639339623Illumina RNA PCR Primer (100% over 31bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG579470.7663245160234916No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA572570.7571995584578505No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT531000.7022249952689079Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA509590.673911177663056No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG456210.6033183900030669Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA430990.5699660088718391No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG367320.4857651323204807Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA336280.4447160478512775No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT326230.4314253487882783Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT324320.4288994547374994Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT304670.4029131625396952Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA290000.3835127092805712No Hit
AGCCGAGTGATCCACCGCTAAGAGTCGGATCGTCGGACTGTAGAACTCTG285380.3774029550844462Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAAC280850.3714122220739601No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT273340.3614805653612114Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT272830.3608061119759249Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG271780.3594175314768056Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTG258220.3414850061738934No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA253940.3358248875679594Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA253520.33526945536831176Illumina RNA PCR Primer (100% over 45bp)
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT252520.33394699775010284Illumina RNA PCR Primer (100% over 31bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT244130.3228515783333305Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACT238240.3150623029620802No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA223710.29584699376950546No Hit
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT216540.2863649726469479Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG216190.28590211248057473Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC213270.28204053623540487Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG208760.2760762523772829Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT195370.2583685448694662Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT191020.25261585423025756Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGGGCCCCCCTCGCGGGGATCGTCGGACTGTAGAACTCTGAACGTGT180020.23806882042996008Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT174920.23132428657709486Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT173120.22894386286431886Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG172970.22874549422158755No Hit
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT172350.2279255704982981Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACG166420.22008339682231948Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG165010.218218731580645No Hit
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG161180.21315371890290505Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT152640.2018599308434013Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC150520.1990563206927985Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG148790.1967684690132972No Hit
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT147760.19540633766654206Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT134440.17779120219199995Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA132830.17566204542668368No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC132300.17496114288903297Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA129180.17083507512022134No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC127820.16903653275945726Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA127630.16878526581199757No Hit
GGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTG124540.16469887177173217Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT119360.15784854130941026Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT116310.1538150455738732Illumina RNA PCR Primer (100% over 31bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT110210.14574805410279912No Hit
CGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAAC108600.14361889733748287Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGGATCGCGGACTGTAGAACTCTGAACGTGTAGATCTC107890.14267995242855455Illumina RNA PCR Primer (96% over 32bp)
CGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTC104970.13881837618338466Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG102950.13614701179460276Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCGAGTGATCCACCGCTAAGAGTCGATCGTCGGACTGTAGAACTCTGA102250.13522129146185655Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG101720.13452038892420587Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC101650.13442781689093122No Hit
TCTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG97730.1292437830275525Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA96850.1280800203235287No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT96610.12776263049515854Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAACCCAGCAGACAATGTAGCTGATCGTCGGACTGTAGAACTCTGAACGT92210.12194381697503953Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGGGCCCCCCTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG88410.11691847802584585Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGATCGTCGGACTGTA87040.1151067110888997No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG84470.11170799501010291No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG83300.11016071959679855Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAAAGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA80080.105902406066166No Hit
GGCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTA78650.1040112916721273No Hit
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGT77430.1023978933779125Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)