FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7953312
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA3669244.61347423563919No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3594794.519865434676774Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2771153.484271709697796Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2716393.415419890480846Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2218242.7890770536853076No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1808632.274058907785838Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA985931.2396470803609867No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA888831.1175595776954306Illumina RNA PCR Primer (100% over 45bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT886741.1149317416442357Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA853621.0732887129286515No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC756860.9516287051230985No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT743090.9343151632929779No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG742320.9333470131688535Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT740060.9305054296876571Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA597200.7508821482169944No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG566530.7123195971690788Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG521450.6556388080839781Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT498590.6268960654378956Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT492840.6196663729525511Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA489620.6156177451607582No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT326500.4105207993852121Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA313000.3935467387674468No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG290890.3657469994890179Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG285900.3594728837495625Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT278220.3498165292647893Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC270880.34058766964001913Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG259600.3264048989905086No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG259170.32586424372638717Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT251170.31580554113808185Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA242270.3046152345085922Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT239530.3011701288720976Illumina RNA PCR Primer (100% over 31bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT220130.2767777750954571Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA219720.27626226658780645No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG214310.26946006896246494Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA212770.2675237687142162No Hit
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT207210.26053297041534396Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA206300.2593887929959242No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT201160.25292607658293803Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC200550.2521591005105797Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT194960.2451305820770014Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC187500.23575084191340664Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG183870.23118670561396307Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG182080.22893607090982976Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT171780.21598549132738665Illumina RNA PCR Primer (100% over 31bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG168920.21238950515206748Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC168670.2120751706961829No Hit
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG163180.20517238604495835Illumina RNA PCR Primer (100% over 36bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA162600.20444313010730625No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT161610.20319836566200347Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA160320.20157639986963924No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG160280.20152610635669768Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG160250.20148838622199156Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACG158070.19874738976667833Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG154880.19473648210959157Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG150510.18924191582072977Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG148770.18705414800777337Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG147490.1854447555936445Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG143060.17987474903537043Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG141630.17807675594771086Illumina RNA PCR Primer (100% over 43bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT141090.17739779352300022Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT140890.1771463259582926Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT133020.16725107728704722Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA127460.160260278988175No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG126610.15919154183816753Illumina RNA PCR Primer (100% over 40bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT126080.15852515279169233Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC125680.15802221766227706No Hit
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT123700.15553268877167148Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC121730.1530557332593013Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT118610.1491328392498622Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTGT117900.14824012939515008Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT117210.14737256629690876Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC115730.14551170631807228Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT115020.14461899646336018Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT114770.14430466200747563Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC113810.14309761769687898No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT111930.14073382258862724Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT111840.1406206621845088Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG111780.14054522191509652Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT111170.1397782458427382Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT105950.13321494240386897Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG105010.1320330448497431Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT102860.12932976852913605Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC101440.12754434881971183Illumina RNA PCR Primer (100% over 32bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG100430.1262744376179383Illumina RNA PCR Primer (100% over 33bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC98740.12414953669615879Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA97860.1230430794114452No Hit
GGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG97300.12233897023026383Illumina RNA PCR Primer (100% over 43bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA95350.11988716147436437No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC94980.11942194647965526Illumina RNA PCR Primer (100% over 39bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG94620.11896930486318152Illumina RNA PCR Primer (100% over 36bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG94260.11851666324670779No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG92330.11609000124727911Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGTA90120.11331128465725977Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA89990.11314783074019982No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG85360.10732635661721808Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT83230.10464822705308179Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT83100.10448477313602182Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA83020.10438418611013876Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGTAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT82690.10396926462837117Illumina RNA PCR Primer (100% over 35bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA82110.10324000869071903No Hit
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG79720.10023497129246282Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)