FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003293522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447262
Sequences flagged as poor quality0
Sequence length126
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA3247095.96095800055147No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2439024.477515493104609No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC2309964.240589125325714No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2297064.216907503255764No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAA1630162.9926227157790466No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC1210412.2220521061773786No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1013351.8602923817506851RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC992021.821135095025721No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC946291.7371846626800767RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA705701.295513232152226RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC637971.1711755373617059RNA PCR Primer, Index 12 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC588091.0796065986912322No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA585731.075274146901691No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT451100.8281224585856161RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA420080.771176418538341RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC406820.7468339139920203No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG391770.7192053549104118No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA384940.7066669457059345No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC348340.6394772272749135No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA296550.5444019399103623RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA284540.5223541661847731No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC276850.5082369821756324No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC268250.4924492341289991No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG267930.49186178303889183RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC229100.4205782648236857No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC225800.41452017545695435No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA203800.3741329130120784RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTG192670.3537006297842843No Hit
TCCCTGTGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA189470.3478261188832114No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC179370.32928469385170017No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA174070.31955503517179823No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC164380.30176628184948695No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC158380.2907515739099753RNA PCR Primer, Index 1 (100% over 27bp)
CTCGTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT148220.27210000179906896RNA PCR Primer, Index 12 (100% over 41bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTTGGAATTCTCGGGTGCC147840.2714024036295665No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG146410.26877723157064964RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC137620.2526406844392651No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG129830.23833992196446585Illumina Small RNA Adapter 2 (100% over 21bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG119720.21978013908638874RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA116240.21339160848147198RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA115050.21120702474013553No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA114870.21087658350195018No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG112790.20705815141625278RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG111970.20555280799785286RNA PCR Primer, Index 1 (100% over 29bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG108160.198558468456263No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC104600.1920230750788194No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA104550.19193128584599015RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG102620.1883882214587806RNA PCR Primer, Index 12 (100% over 37bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG101480.18629542695027337RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA101130.18565290232046852RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTTGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC99150.1820180487004297No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA98800.18137552407062482No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT98130.18014554835071273RNA PCR Primer, Index 12 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG97640.17924601386898592No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA97240.1785117000063518RNA PCR Primer, Index 1 (100% over 23bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTAT96380.1769329252016885RNA PCR Primer, Index 12 (100% over 48bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGGAATTCTCGGGTGC94350.17320628234882038No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC93220.17113184568687903No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC91350.16769892837906458No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT90280.16573463879651834RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA89810.16487182000792325RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC89570.16443123169034277RNA PCR Primer, Index 1 (100% over 27bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA89020.16342155012922088RNA PCR Primer, Index 12 (100% over 40bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG88740.162907530425377RNA PCR Primer, Index 12 (100% over 45bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG88460.16239351072153313No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG85710.15734510291592363RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT84940.15593154873035298RNA PCR Primer, Index 1 (97% over 35bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA83960.15413247976689942RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA83610.15348995513709457No Hit
TCCCTGTCGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC82110.15073627815221666No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC81030.14875363072310455RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG79810.14651397344207054RNA PCR Primer, Index 1 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC78480.14407237984881213No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC76730.1408597566997879RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTTTGGAATTCTCGGGTGC76410.1402723056096806No Hit
TCCCTGGTCTAGTGGCTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG74710.13715147169348565No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT73000.13401227993072481RNA PCR Primer, Index 1 (97% over 35bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA72660.13338811314748583No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG68170.12514544003941797RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC68020.12487007234093019RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC67100.12318115045687172No Hit
TCTGACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT66670.12239176305454005RNA PCR Primer, Index 12 (100% over 41bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA66590.12224490028201324RNA PCR Primer, Index 1 (100% over 28bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT64520.11844482604288173RNA PCR Primer, Index 12 (100% over 43bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC63680.11690276693135009No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA63570.11670083061912573RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGTGGAATTCTCGGGTGC62080.11396551148081366No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG61780.11341477608383808RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA61580.11304761915252103RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA61070.11211136897766254RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA60980.11194614835856986RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG60280.11066109909896019RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTAT59480.10919247137369195RNA PCR Primer, Index 12 (100% over 48bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA58970.10825622119883346No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA58260.10695281409265793No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA56650.10399720079555565No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA56210.10318945554665812No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA55970.10274886722907764No Hit
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG55780.10240006814432646RNA PCR Primer, Index 12 (100% over 45bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC55450.10179425920765332RNA PCR Primer, Index 12 (100% over 44bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG55450.10179425920765332RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA55090.10113337673128263RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA54750.10050920994804362RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA54720.10045413640834608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)