FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003293523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447262
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA2986685.482901318130099No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2230064.093909931264551No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2153523.9533989736495143No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG2130863.911800093331292No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC1549882.8452459235483807No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA1133102.0801275943767714No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT922301.693144188768596Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG918021.685287030438411No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG914291.678439553669348Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT684021.2557134207974574Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC543230.9972532989968171No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA537740.987174841232164No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA531140.9750586624987012Illumina RNA PCR Primer (100% over 45bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT429110.787753553987306Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT391820.7192971441432412Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG382450.7020958419110371No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT370260.6797176269472627No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC353640.6492068859548155No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA322950.5928666548442134No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT275920.5065297024450082Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA261510.4800760455436144No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG259830.4769919273205511Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA247930.4551460899071864No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA243830.44761937281518677No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA210220.3859186505073558No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG206160.37846536480161963No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT191160.3509285949528405Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAA180650.3316344982121293No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA178310.3273387621157198No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA172690.31702165234571056No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA159160.29218348594211185No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG153630.28203159679119527Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA144110.2645549268605035No Hit
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG140150.25728521962042583Illumina RNA PCR Primer (100% over 36bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG136910.2513372773330895Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAGA135950.24957492406276768No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG126840.23285092584127584No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC122640.22514063028361772Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG108960.2000270961815312Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA104800.19239023201013647No Hit
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG104630.19207814861851696Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG104300.19147233968184382Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT101120.18563454447390268No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA100890.18521231400288807No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT99300.18229341639891747Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA97840.17961317080030298No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT95600.17550101316955197Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG95500.17531743470389344Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT95270.17489520423287883Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC94270.17305941957629356Illumina RNA PCR Primer (100% over 32bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC91480.16793758038442066No Hit
GAGCGCCGAATCCTAACCACTAGACAACAGGGAGATCGTCGGACTGTAGA90610.16634044773319148No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG87380.16041086329242105No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT86510.15881373064119186No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT84960.1559682644234847Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT84110.1544078474653872Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT84070.1543344160791238Illumina RNA PCR Primer (100% over 31bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG83900.1540223326875043Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG83450.15319622959204093No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG83300.15292086189355314Illumina RNA PCR Primer (100% over 40bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG83140.15262713634849948Illumina RNA PCR Primer (100% over 43bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA82510.15147059201485075No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT82470.15139716062858735No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC81970.15047926830029473Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG81040.14877198856967042Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT80410.1476154442360217Illumina RNA PCR Primer (100% over 35bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG78540.14418252692820724Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC77320.1419428696471732No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT77180.14168585979525125Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACGACAGGGAGATCGTCGGACTGTAGA75760.13907904558290018No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG74500.13676595691560275Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA74270.13634372644458814Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA72920.133865417158198No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC70910.13017548999846162Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAGCCACTAGACCAGGGAGATCGTCGGACTGTAGAACT70910.13017548999846162No Hit
AAGCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAG69980.1284682102678373No Hit
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC65750.1207028411704816Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG65080.11947286545056948Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT61630.11313940838535029Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGAA61410.11273553576090153No Hit
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT60790.11159734927381866Illumina RNA PCR Primer (100% over 38bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG60520.11110168741654064No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT59060.10842144181792614Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAG58840.10801756919347738No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT57980.10643879438881405Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG57740.10599820607123359Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAG56910.10447450480626781No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT56900.10445614695970196Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG56790.10425421064747759Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG56590.10388705371616053Illumina DpnII expression Sequencing Primer (95% over 24bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG56280.1033179604726191Illumina RNA PCR Primer (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)