FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003293525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5046503
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA2637095.2255789801373345No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2118694.198332984246715No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1957793.879498337759831No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1603413.177269487405437Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1248012.4730194354387582Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1078222.1365686298016664Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT920651.82433261210783Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC572131.1337157631730328No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT527581.0454368104011826No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT432870.8577622959899162Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG427070.8462691887828065Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT409790.8120276555864527Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA367920.7290593109723704Illumina RNA PCR Primer (100% over 45bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC299840.5941540112034016No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT285490.5657184787168461Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA248030.49148885872058334No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG242570.4806694853842354Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG237440.4705040302165678Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA221500.4389178010990978No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG221190.4383035143345798No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC209910.4159514023869599No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT207230.41064079422919203Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT193960.38434535756740856No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA191600.37966885187623983No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA173600.34400058813003775No Hit
AGCGCCGAATCCTAACCACTAGACTACCAGGGAGATCGTCGGACTGTAGA163980.3249378827279009No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT161620.32026137703673213Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA156820.3107498400377449No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG150000.2972355312183506Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG148510.29428299160824833Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA142760.28288896291154486No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT139770.2769640679892591Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC137840.27313963748758296Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT130330.25825804522458423No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG126770.2512036552836687Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA122940.24361424138656015No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT120720.2392151555245286Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC116830.23150684741493266Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG114890.22766260121117535Illumina RNA PCR Primer (100% over 40bp)
GAGCGCCGAATCCTAACCACTAGACTACCAGGGAGATCGTCGGACTGTAG114190.22627550206548971No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA113980.22585937232178405No Hit
AGGGCCCGGCTCGCGTCCAGGATCGTCGGACTGTAGAACTCTGAACGTGT111490.2209252625035594Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG110620.21920129642249297Illumina RNA PCR Primer (100% over 36bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA110120.2182105113184318No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG102590.20328928765127058Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT100300.19875149187467042Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT98960.1960961877957865Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT94480.18721875326339843Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC94360.18698096483842377Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG92890.1840680566324839No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT92620.1835330326762909Illumina RNA PCR Primer (100% over 31bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA92140.18258187897639216Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG91690.18169017238273713Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG88360.17509154358968973Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT85530.16948369990070353Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA76200.1509956498589221No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG74060.1467550896135403Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA73370.1453878061699359No Hit
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT72840.14433757395963107Illumina RNA PCR Primer (100% over 38bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG72110.14289102770770173Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA71910.14249471366607727No Hit
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG71780.14223710953902138Illumina RNA PCR Primer (100% over 43bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG71050.14079056328709208Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT70220.13914586001435053Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG69900.13851175754775139Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC68760.13625276751049192No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG68700.13613387329800458Illumina DpnII expression Sequencing Primer (96% over 26bp)
GACCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG68180.13510345678978097Illumina RNA PCR Primer (100% over 36bp)
ACTCGGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT67640.1340334088773949Illumina RNA PCR Primer (100% over 35bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT67190.13314170228373987Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG67100.13296336096500885Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC66440.1316555246276481Illumina RNA PCR Primer (100% over 39bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT66210.13119976347977996Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT65240.1292776403779013No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG65050.12890114203835804No Hit
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT64250.12731588587186018Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACG64220.12725643876561651Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG63050.12493800162211337Illumina RNA PCR Primer (100% over 36bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT62520.12388776941180853Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGTA60480.11984536618723897Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG60260.11940942074145205Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG59380.11766563895830438No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT59110.11713061500211136Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC58630.11617946130221264No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA58560.11604075138764408No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT58030.11499051917733924Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC57920.11477254645444579No Hit
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG55820.11061124901738886Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT52940.10490432681799654Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG52650.10432967145764106Illumina RNA PCR Primer (100% over 33bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT51510.1020706814203816Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG51310.10167436737875714Illumina RNA PCR Primer (100% over 40bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC51240.10153565746418858Illumina RNA PCR Primer (100% over 39bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT50750.10056468806220861Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC50750.10056468806220861Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTGT50590.10024763682890904Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)