FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607757
Sequences flagged as poor quality0
Sequence length126
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG7830929.097515183107516Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT5640986.553368084159439Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4660755.414592907304423Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2521932.9298341019617538Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1822602.117392486799987Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1391021.6160075150820359Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1023801.1893923120738654Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1016011.1803423354074702Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA993031.1536454851130207No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG777950.903777836665231Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA743510.8637674135085366Illumina RNA PCR Primer (100% over 45bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG706010.8202020572839126Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT692750.8047973473228857Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG642630.7465707965501349Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA592330.6881351320675061No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT504850.5865058690667034Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG462010.5367368061156931Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT450460.5233186763985089Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT449920.5226913352688743Illumina RNA PCR Primer (100% over 38bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA438570.5095055541182215No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG412530.47925377075584263Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC404750.4702154115177741Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT389690.452719564457965Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG387740.4504541659342846Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG357400.41520688839148223Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG344430.40013908385192565Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG327600.38058695197831444Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG327230.3801571071302315Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG317390.3687255576568902Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT307370.35708489447367064Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT297980.34617612927502484Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG280060.3253576977138179Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA275030.319514131265555Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT272660.3167608007521588Illumina RNA PCR Primer (100% over 31bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG268970.3124739696996558Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT260140.30221578048729764Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACG253450.2944437209368248Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT248690.2889138250533792Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG234270.27216149340647044Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG229990.26718923408270007Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA229730.26688718094620933No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT224850.26121787592284496No Hit
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC217390.25255127439122643Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT215430.2502742584392194Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT208080.24173544861919313Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG201560.23416088535027185Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGTA194870.22638882579979897Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT190980.22186964618076463Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC185510.2155149128861328Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG183600.21329598407575867Illumina DpnII expression Sequencing Primer (96% over 26bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT179280.20827725503868194Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC178260.2070922773493722Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT171890.19969197550534942Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG168470.1957188150176637Illumina DpnII expression Sequencing Primer (95% over 24bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT164050.1905839116973214Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT161300.18738911890751564Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG156700.18204510187729508Illumina RNA PCR Primer (100% over 36bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG154660.17967514649867555Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT148660.1727046895027357Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT146000.16961445356786908Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT144150.16746522932745428Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT143530.16674494877120719Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG141770.1647002813857315No Hit
ACAGATGTCCAGCCACAATTCTGATCGTCGGACTGTAGAACTCTGAACGT141280.16413102739772975Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG138730.16116858317445532Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC135780.15774144181811825Illumina DpnII expression Sequencing Primer (100% over 26bp)
CATTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG130370.15145641309344582Illumina RNA PCR Primer (100% over 40bp)
AGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA129960.15098009853205663Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA129670.1506431931105862No Hit
CAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTGT129450.15038760968740172Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG126180.14658871062461454Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG123430.14339391783480876Illumina RNA PCR Primer (100% over 37bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG122570.14239481899872405Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC122180.14194173929398798Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC121480.14112851931112833No Hit
TCCTCAAGGAGCTTCAGTCTAGGATCGTCGGACTGTAGAACTCTGAACGT121340.1409658753145564Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC120160.13959501877202157No Hit
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG118550.13772461281144438Illumina RNA PCR Primer (100% over 33bp)
CAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG115930.13468084658988397Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT114960.13355395604220704Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT114400.1329033800559193Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT113350.13168355008162985Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT112170.13031269353909503Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT110460.12832611329525218Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT110090.12789626844716923Illumina DpnII expression Sequencing Primer (96% over 28bp)
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG110060.12786141616218952Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA108570.12613041934153113Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG107640.12504999850716045Illumina RNA PCR Primer (100% over 36bp)
GGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG107220.12456206651744468Illumina RNA PCR Primer (100% over 43bp)
CAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTGT106870.12415545652601485Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG104720.12165770943580309Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG102840.1194736329104086No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT102640.11924128434387728Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG99570.11567473384762139Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT99200.11524488899953844Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT97890.11372300588875825Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT96540.11215465306467179Illumina RNA PCR Primer (100% over 44bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA94030.10923867855470362No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC92900.10792590915380162Illumina RNA PCR Primer (100% over 39bp)
TCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT92750.10775164772890314Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT92640.1076238560173109No Hit
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG92340.1072753331675139Illumina RNA PCR Primer (100% over 43bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA91380.10616006004816353Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA90760.10543977949191642Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC90340.10495184750220063Illumina RNA PCR Primer (100% over 39bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC89090.10349966896137984Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC88850.10322085068154224Illumina RNA PCR Primer (100% over 32bp)
TCCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG87860.10207072527721216Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG87600.10176867214072144Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)