FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7236972
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA4443536.140040337312345No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT2528033.493215118146097No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1866732.5794351560293447Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1712802.366735701063926No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1494272.0647723937580524Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA930831.2862147317966686No Hit
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC863521.1932062193967312Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC844001.1662336126214112No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA764611.056533036192485No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT728161.0061666674957428Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT709660.9806034899679038Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT639500.8836568664352993Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA559650.7733206650516266Illumina RNA PCR Primer (100% over 45bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG542380.7494570933810438Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT500430.6914908610949442Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTAGAAC489270.6760700469754477No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG399710.5523166318731094No Hit
GCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAACT384880.5318246360494416No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA357360.49379768223505627No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA334750.4625553339158974No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT329620.45546673387709663Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG306640.423713122007381Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT296470.4096602833339689No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT289870.40054044702673997Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT289770.4004022676887516Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT288080.398067036876749Illumina RNA PCR Primer (100% over 31bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG276930.3826600406910515Illumina RNA PCR Primer (100% over 40bp)
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT262030.36207131933079195No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA258630.35737322183918907No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC254340.3514453282394902No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG246750.3409575164861768No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC241710.3339932778515656Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAACTC236660.32701522128315547Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC232350.3210596918158589Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT226950.31359800756448963Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA223030.3081813775153476No Hit
CAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTAGAACT211940.292857288932443No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA192510.26600904356131266No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA186660.257925552288996Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT182050.2515554848077345Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG179270.24771409921165924Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG174440.24104003718682346Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT170400.23545759193209534Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG163940.22653120669804994Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC162160.2240716144818579Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG161900.22371234820308825Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACTC161080.22257927763158405Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG156740.21658229436289098Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT154300.213210718515976Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC153870.21261654736262625No Hit
CGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAAC152200.2103089524182213No Hit
CGCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTAGAA149510.20659192822633554No Hit
GCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAACT142340.19668446969257308No Hit
AGCCGACATCGAAGGATCAGATCGTCGGACTGTAGAACTCTGAACGTGTA142320.19665683382497542Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG140550.19421105954258217Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC136290.18832461974427978No Hit
GCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAACT135240.18687373669540244No Hit
CAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGTG134140.18535376397753092Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT126710.17508703916499885Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA119960.16575993385078733No Hit
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT117950.1629825291572221Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA117170.16190473032091324No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT115720.1599011299200826Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG109600.1514445544351975Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC108280.14962058717375168Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGTCGCGCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT107080.14796243511789187Illumina RNA PCR Primer (100% over 35bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT106630.14734062809694443Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT104980.14506066902013715Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA104330.14416250332321306No Hit
GGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT102750.14197926978299763Illumina RNA PCR Primer (100% over 38bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC102270.14131600896065372Illumina RNA PCR Primer (100% over 39bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT101140.13975458244138572Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG100210.13846951459809434Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG99480.13746080543077963No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG97730.13504266701598405Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC96080.13276270793917677Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG94270.1302616619215882Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAACT92430.12771916210260315No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT91170.1259781024439503Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG91140.1259366486425538Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA89510.12368432543334422No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA87130.12039565718922224No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT86800.11993966537386079Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAAC84290.11647136399035399No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG83550.11544883688924042No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA83490.11536592928644743No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT82250.11365250549539227Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT82050.11337614681941563Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA81620.11278197566606586No Hit
GCCCGGCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG80990.11191144583673944Illumina RNA PCR Primer (100% over 37bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT77840.1075587966901074No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA77460.1070337152057518No Hit
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG77260.10675735652977515Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT76190.10527883761330015Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT75060.10371741109403215Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT74650.10315087580828003Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGACAGCTACAGGTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT74190.10251525085353376Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAACTC73620.10172762862700035No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC73480.1015341775538167Illumina RNA PCR Primer (100% over 32bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG72500.10018002004153119Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC72370.10000038690214637Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)