FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7272648
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA91474112.577825848301746No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1960922.6962943896088465No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1959492.694328118176488No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT1868952.569834261193447No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1821812.505016054674996Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1446031.9883129226108565Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1296711.7829956846529629No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1143561.5724121392923185No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1093381.5034138872113707Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT995181.3683874154228282Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT949681.305824233484145Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG902731.24126727981335Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT828471.1391586668294684Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA763041.0491914361866546No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA701080.9639955075510322No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA657740.904402357985702No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT623050.8567030880636598Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC545790.7504694301167882No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC538230.7400743168100532Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC522950.719064087798557Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG419590.5769425386736715Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT395000.5431309201270293Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT379940.522423194412819No Hit
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG365690.5028292308386162Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC362830.4988966879738989Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG349360.4803752360900733Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA347860.4783127136085783No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA331770.4561887224570748No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT329220.4526824342385332Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT310610.42709340531811796Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA292400.4020543823927681No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG290490.39942810376633103No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT273570.3761628501750669Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGT261830.360020174219899No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA254840.3504088194561321No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC248590.34181497578323605Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG240460.3306361039335329Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT231320.3180684669462897Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAACT208650.28689687717596124No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG205290.2822768268174123Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAAC205040.2819330730704965No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG190940.262545361744443Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA189740.260895343759247No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA185550.25513403096093745No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC185140.2545702748159955No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTG175920.24189263662973925No Hit
CAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGTG175260.24098512673788147Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT175150.24083387508923845Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA173570.23866135140873038No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG167570.23041126148275015Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTC164680.22643746816840304Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA164240.22583246157383113No Hit
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT164040.22555745857629847No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG158360.21774737344637057No Hit
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG156090.21462608942437472Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT152870.21019854116409867Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA151350.20810851838285035No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT145850.20054593595070186Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT141540.19461962135387276Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC140630.19336835771509908No Hit
GCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAACT138420.19032957459236305No Hit
CGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAAC137390.1889133091550698No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT137300.1887895578061801Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG127950.1759331676715276Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG122320.16819183329098286No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG120740.16601930961047476Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT119860.164809296421331Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT119800.1647267955220712Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG118890.16347553188329753Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT118260.16260927244106962Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAACT117110.16102800520525673No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT114540.15749421668696187Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG112730.1550054395592912Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC109080.14998663485431993Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC108890.14972538200666388No Hit
AGTAGTGCTTTCTACTTTATGGATCGTCGGACTGTAGAACTCTGAACGTG108810.1496153808076508Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTGCTTTCTACTTTATGGGGATCGTCGGACTGTAGAACTCTGAACGTGT107990.14848786851776685Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG106760.1467966000829409Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA106560.1465215970854082No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC106400.1463015946873821Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT105420.144954079999472Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC104620.1438540680093413No Hit
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGT104620.1438540680093413Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA104490.14367531606094508No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT104240.14333156231402924Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT102450.1408702854861118Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTAGAA100690.13845025910782427No Hit
TATCAGATATTTTATTGTCATCAAGTAAGATCGTCGGACTGTAGAACTCT97800.13447646579347716Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA96650.13289519855766427No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG94960.13057142322851317Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC94900.13048892232925338Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT94140.12944391093862923Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT93240.1282063974497322Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA92350.12698263411071178No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG92020.1265288791647829Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACT88950.12230758315265637No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA88370.1215100744598116Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT87980.1209738186146229Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG87330.12008005887264171Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA85760.11792128534201021No Hit
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT85600.11770128294398409Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA80050.11006994976245242No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG79960.1099461984135627Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAACTC79660.1095336939172637Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG79070.10872243507454231Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGGGGCTCGTCCGGGATGATCGTCGGACTGTAGAACTCTGAACGTGTAG77310.10630240869625479Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAACT76800.10560115105254647No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG75990.10448738891253916Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG75730.10412988501574667Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAAC74910.10300237272586271No Hit
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT73080.10048609529843876Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG72960.10032109349991913Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)