FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7971181
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA4261155.345694696933867No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA4161335.2204685855207655No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA2869873.600307156492871No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2485723.1183835870744874Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1915422.4029312594959267No Hit
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG1201861.507756504337312Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1170101.4679129729960967No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1060081.329890765245451Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA920121.1543082511863674No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT856341.0742950134992544Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG781480.9803817025356719Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT781440.9803315217657208Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATTCGGTTCTACAGGGTAGATCGTCGGACTGTAGAACTCTGAACGT749080.9397352788752382Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT710240.8910097512526688Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT686030.8606378402397336Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG644390.8083996587205835Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT598510.7508423155866114Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT588440.7382093067514086Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT573070.7189273458976781Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA523880.657217544050248No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT505710.6344229292999368Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC486360.6101479818360668Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA478680.6005132740054453No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA457370.5737794688139687No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA452450.5676072341099769No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA450180.5647594754152491No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT444530.5576714416596487Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG433040.5432570154911801No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC426600.5351779115290445No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA416180.5221058209567692Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG411990.516849385304386Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA384450.4822999251930172No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT373620.4687134817287426Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG368790.4626541537571409Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG314170.3941323123888418No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA306560.3845854209056349No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT291460.36564218024907474Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG286740.3597208493948387No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC280620.35204319159231234Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG264570.3319081576494123Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG252760.31709228532133443Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCGATCG240500.3017118793313061No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG219910.2758813279989502Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT216150.27116433562354186Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT215260.2700478134921287Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA204980.25715135561468244No Hit
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT200760.2518572843848358Illumina RNA PCR Primer (100% over 31bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT186050.2334033062352994Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT183710.23046773119315694Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG169380.21249047035815644Illumina DpnII expression Sequencing Primer (96% over 29bp)
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGT168210.21102268283708525No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC166010.20826274048977184Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT164210.20600460584196995Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA163950.20567843083728748No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG163150.2046748154382644No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT159180.1996943740206125Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT153810.19295760565467024No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT151350.18987148830267433Illumina RNA PCR Primer (100% over 31bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG150040.1882280680867741No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATC143320.17979769873498044No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT141980.1781166429416168Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG141560.17758974485712972Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT139580.17510579674454765Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT137330.1722831284347953Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC134910.16924719185275056No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA133250.16716468989977773No Hit
CCACTGAACCACCAATGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG131960.16554636006885304Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC131620.16511982352426824No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC126440.15862141381559394Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA125770.15778088591891212No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG124480.1561625560879875Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT124180.15578620031335383No Hit
ACAAATTCGGATCTACAGGGTAGATCGTCGGACTGTAGAACTCTGAACGT123780.15528439261384228Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA122330.153465339703113No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA120500.15116956947784776No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTG120090.15065521658584846No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT118080.14813363289580303Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA118050.14809599731833964No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG117120.14692929441697536Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG115930.14543641651092856Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG110170.13821038563796256No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC109300.13711895389152498No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA107620.13501136155357657No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT107140.13440919231416273No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT106470.13356866441748091Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG105500.13235178074616546No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT105420.13225141920626315Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA104940.13164924996684932No Hit
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC99870.12528883737554072Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG99640.1250002979483216Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT95890.120295850765401Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA94870.11901624113164662No Hit
CACAAATTCGGTTCTACAGGGTAGATCGTCGGACTGTAGAACTCTGAACG93910.11781190265281893Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA91450.11472578530082306Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACCCAGCAGACAATGTAGCTGATCGTCGGACTGTAGAACTCTGAACGT88790.11138876409907139Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAAAGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA88090.11051060062492622No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT87450.10970770830570778Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG86880.10899263233390384No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC86650.10870409290668472Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG86530.10855355059683128Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAATGTGATAACCGCTACACTACGGAAACGATCGTCGGACTGTAGA82880.10397455533878858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)