FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588681
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA153189417.836196268088198No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2565602.9871874389094204No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2405372.800627942753957No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2218332.5828529433099217Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG1827422.127707386035178No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1280171.4905315496058125No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1250821.4563586655506242Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1212011.411171284624496Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1122371.3068013586719545Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1072451.2486783476997225Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1011871.178143652092795No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG992241.1552879889240268No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT986531.1486397038148233Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG828100.9641759893049934No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT810970.9442311339773826Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA756040.8802748640914712No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA714660.832095172704633No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC675750.7867913594648585No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC647310.7536780094638513Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA646710.7529794155819735No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT627140.7301936118013931Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG598060.6963350949930496No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG575180.6696953816307766Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA527670.6143783894174204No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT472180.5497700985750897Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA390000.454086023220562No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA377180.4391594006111066No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC369750.43050847970718664No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA356430.4149996955294998No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA340170.3960678013306118No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT303460.3533254989910558Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA271860.31653288787882566No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG271000.3155315699814675Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA259650.30231650238261265No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG253100.29469018583878015No Hit
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT246690.28722687453405243No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA242850.2827558736900346No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC231400.2694243737775335Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA228160.2656519668153934No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT228080.26555882096447636Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT221080.25740855900923554Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA217150.252832769082936No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA216610.252204034589246No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC213080.2480939739175317No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC212660.24760495820021722No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT207130.24116625125557697Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG199680.23249204388892777Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA192200.2237829068281847No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG192170.22374797713409078Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA190570.22188506011575002No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT184790.21515527238699397Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT176960.20603862222848887Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC176430.20542153096616347Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG175790.20467636415882717Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG175260.2040592728965018Illumina DpnII expression Sequencing Primer (95% over 24bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG167120.19458168256569314No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG165260.192416041531872No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT161740.1883176240915223Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG160530.1869087930964021Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA153520.17874688790979662Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT148270.17263419144336598No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT148250.17261090498063675Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG147660.17192395433012356No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT147020.17117878752278726Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT144270.16797689889751405Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT142390.16578797140096366No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA141310.16453050241358363No Hit
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT137170.1597102046286269Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTA128580.1497086688864099No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA127000.1478690383307984No Hit
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA126190.1469259365902634No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG124630.14510959249738115No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA117340.136621676832566No Hit
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT115970.13502655413561174No Hit
AAAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAAC115700.13471218688876674Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG115420.1343861764105571No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC112570.13106785547163763No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA112410.13088156376980353No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG111570.12990353233517463Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG110340.12847141487732516Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG109340.1273070917408622Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT107370.12501337516203012No Hit
AGGCGAAAGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA106070.12349975508462825No Hit
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG104090.12119439527443153Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG104060.12115946558033765Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT103880.12094988741577432Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG103850.12091495772168044No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT103740.1207868821766695Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG102440.11927326209926763Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG101920.11866781406830687Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC98850.11509334203936553Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT98820.11505841234527164Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGACAGCTACAGGTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT93940.1093765154393323Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT92570.10778139274237802Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG92140.10728073379369894Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGT91590.1066403560686443No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC91510.10654721021772726Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA89710.1044514285720939No Hit
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC89340.1040206290116026Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAAGGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA89130.10377612115294538No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA87230.10156390719366572No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA86500.10071395130404773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)