FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003293684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6014989
Sequences flagged as poor quality0
Sequence length126
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2957004.916052215556836RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2165693.6004887124481852RNA PCR Primer, Index 3 (100% over 50bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1626652.704327472585569No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1370072.277759776451794RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1168011.9418323125777952RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1066951.773818705237865RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1002951.6674178456519206RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTAT825531.3724547127185105RNA PCR Primer, Index 3 (100% over 48bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG785971.3066856813869485RNA PCR Primer, Index 3 (100% over 45bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA632301.05120724244051RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT602091.0009827116890821RNA PCR Primer, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC587790.9772087696253475No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT585720.9737673668231148RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA559930.9308911454368413RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA540300.8982560067857147No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA474130.7882474930544345RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC444530.7390370954959352RNA PCR Primer, Index 3 (100% over 44bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC435920.7247228548547636No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC408120.678504981472119No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT392800.6530352757087337RNA PCR Primer, Index 1 (100% over 30bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC376170.6253876773506984No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC368040.6118714431564214No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC351580.5845064720816614RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG350240.5822787040840806RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC321370.5342819413302335RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT307550.5113060057133937RNA PCR Primer, Index 3 (100% over 43bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT278600.4631762418850641RNA PCR Primer, Index 3 (100% over 41bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC277820.4618794814088605RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA274320.45606068440025405RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA268560.4464846070375191RNA PCR Primer, Index 3 (100% over 36bp)
CCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT267330.44443971551735173RNA PCR Primer, Index 3 (100% over 41bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA262270.43602739755633796RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG244220.40601903012623963RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC237310.3945310623178197RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG234140.3892608947414534RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC217790.36207880014410665No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG209280.3479308108460381RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA208880.34726580547362595RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT202410.3365093435748594RNA PCR Primer, Index 3 (100% over 43bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA194360.32312611045506484No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA182080.30271044552201176RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA181680.3020454401495996RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC179610.2986040373473667RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC177760.2955283874999605RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC176860.29403212541203316No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA172840.287348821419291RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA172010.28596893527153583RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA163950.27256907701743094RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA162590.27030805875122965No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA161330.26821329182813136No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG156930.2608982327315977RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC155000.2576895818097091RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA153520.2552290619317841RNA PCR Primer, Index 1 (100% over 28bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC152420.25340029715765067RNA PCR Primer, Index 3 (100% over 39bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA144880.2408649458876816No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG142370.23669203717579532RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC134200.22310930244427712RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT132010.2194683980303206RNA PCR Primer, Index 3 (100% over 43bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA131840.21918577074704543RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC128150.21305109618654333RNA PCR Primer, Index 1 (100% over 33bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC124550.20706604783483396RNA PCR Primer, Index 3 (100% over 44bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC122620.2038573969129453RNA PCR Primer, Index 1 (100% over 31bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTA119440.19857060420226869RNA PCR Primer, Index 3 (100% over 47bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC115690.19233617883590476No Hit
TGCAACGAACCTGAGCCACTGATGGAATTCTCGGGTGCCAAGGAACTCCA112530.18708263639384878RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA112280.18666700803609118RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA111480.18533699729126688No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA111230.18492136893350927RNA PCR Primer, Index 1 (100% over 28bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT109820.18257722499575643RNA PCR Primer, Index 3 (100% over 46bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC109010.18123058911662182RNA PCR Primer, Index 1 (100% over 26bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG106910.177739310911458RNA PCR Primer, Index 3 (100% over 45bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA106230.17660880177835736RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA103210.17158801121664563No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA101830.16929374268182368RNA PCR Primer, Index 1 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC96810.16094792525805118RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC96690.16074842364632752RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA94290.15675839141185463RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG92260.15338348914686295RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC88030.14635105733360443No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA87550.14555305088670983RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC87160.14490467064860799RNA PCR Primer, Index 3 (100% over 42bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG85280.14177914539827088RNA PCR Primer, Index 3 (100% over 37bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG84360.14024963304172294RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA82390.1369744815825931RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC80490.13381570606363535RNA PCR Primer, Index 3 (100% over 44bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC79620.1323693193786389No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC79450.13208669209536378No Hit
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCA79410.13202019155812256RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT79410.13202019155812256RNA PCR Primer, Index 3 (100% over 34bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA79320.1318705653493298RNA PCR Primer, Index 1 (100% over 28bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG79310.1318539402150195RNA PCR Primer, Index 3 (100% over 49bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC79100.13150481239450315RNA PCR Primer, Index 3 (100% over 42bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT77400.1286785395617515RNA PCR Primer, Index 1 (97% over 35bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT77300.12851228821864846RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT77190.1283294117412351RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCCGTAGTGTAGAGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC74370.12364112386572942No Hit
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCC73650.12244411419538755RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA71770.11931858894505044RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG71290.11852058249815585RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG69960.11630943963488545No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG69230.11509580483023327RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG68850.11446404972644172RNA PCR Primer, Index 1 (100% over 29bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG68460.11381566948833989RNA PCR Primer, Index 3 (100% over 38bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG67810.11273503575817012RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC65890.10954300997059181No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC64950.10798024734542323RNA PCR Primer, Index 1 (100% over 26bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC64570.10734849224163169RNA PCR Primer, Index 3 (100% over 44bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT62560.10400684024526063RNA PCR Primer, Index 3 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA62460.10384058890215761No Hit
ATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA62400.10374083809629576RNA PCR Primer, Index 3 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC62330.10362446215612364No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC61350.10199519899371386RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG60780.10104756633802654RNA PCR Primer, Index 3 (100% over 37bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA60690.10089794012923382RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)