FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6014989
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2776384.615769039644129Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1755652.918791705188488Illumina RNA PCR Primer (100% over 45bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1518722.52489239797446No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1277312.123545030589416Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1104661.8365120867220208Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1030741.713619093900255Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT861481.4322220705640525Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG724781.2049564845421994Illumina RNA PCR Primer (100% over 40bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG705091.1722215950852113Illumina RNA PCR Primer (100% over 43bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT606801.0088131499492352Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC579550.9635096589536574Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT545320.9066018242094874Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA536280.8915727027929726No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT515550.8571087993677128Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA491150.8165434716505717No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT458450.7621792824558781Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC405260.6737501930593722Illumina RNA PCR Primer (100% over 39bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG404580.6726196839262715No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT372850.6198681327596776Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG367590.6111233121124577No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG344480.5727026267213455Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG341420.5676153356223926No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA341100.5670833313244629No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC332760.5532179693096695Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA295180.4907407145715479Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT275730.458404828338007Illumina RNA PCR Primer (100% over 38bp)
GCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG254660.42337567034619683Illumina RNA PCR Primer (100% over 36bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT252760.4202168948272391Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT252680.4200838937527566Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG247740.41187107740346657Illumina RNA PCR Primer (100% over 36bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT244520.40651778415554873Illumina RNA PCR Primer (100% over 31bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG230220.38274384209181433Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC224540.37330076580356175Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG222390.36972636192684644Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG213340.35468061537602147Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA195200.32452262173713037No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG193710.3220454767248951Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT193560.32179609971024054Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT182160.3028434465964942Illumina RNA PCR Primer (100% over 38bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT176980.2942316270237568Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA176550.2935167462484138No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG172160.2862183122861904Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT171340.28485505127274546Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG163880.27245270107725883Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG162670.27044105982571204No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT161690.2688117966633023Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT154200.2563595710648848Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG152400.25336704688903006Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT149630.24876188468507587Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA148580.247016245582494No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA146720.2439239706007775No Hit
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC144910.24091482129061248Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG143230.23812179872648148Illumina RNA PCR Primer (100% over 34bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT140980.23438114350666311Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG130010.21614337116825982Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG129390.21511261284102098Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA127800.2124692164856827No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG118030.19622646026451587Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC115420.19188730020952657Illumina RNA PCR Primer (100% over 39bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA112330.18675013370764268Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT111470.18532037215695657Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT108280.18001695431196965Illumina RNA PCR Primer (100% over 38bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT106940.17778918631438895Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA106550.17714080607628707Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT104990.1745472851238797Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTGGCTCAGGTTCGTTGCAGATCGTCGGACTGTAGAACTCTGAACGT103750.17248576846940203Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA102580.17054062775509649No Hit
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC101600.1689113645926867Illumina RNA PCR Primer (100% over 42bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA101290.16839598542906728No Hit
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG100870.16769772978803452Illumina RNA PCR Primer (100% over 40bp)
TGGGGGCTCGTCCGGGATGATCGTCGGACTGTAGAACTCTGAACGTGTAG98090.16307594244977008Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC97630.1623111862714961Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC95670.15905265994667653No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT95300.1584375299771953Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC94660.15737352138133587Illumina RNA PCR Primer (100% over 41bp)
ACTGAGGGGCCTCAGACCGAGCTGATCGTCGGACTGTAGAACTCTGAACG91260.15172097571583257Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAATTCGGTTCTACAGGGTAGATCGTCGGACTGTAGAACTCTGAACGT90890.15110584574635133Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTTCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA87580.14560292628964078Illumina RNA PCR Primer (100% over 45bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG86050.14305928074016427Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG83280.13845411853621012No Hit
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT82340.13689135591104157Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG78860.13110580917105583Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC78540.13057380487312614Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTTCTGACTCCAAGTCCAGTGATCGTCGGACTGTAGAACTCTGAACGT77270.12846241281571752Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT77130.1282296609353733Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT76070.1264673966984811Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC74620.12405675222348703Illumina RNA PCR Primer (100% over 39bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG74430.12374087467159126Illumina RNA PCR Primer (100% over 37bp)
AGGCGAACGTGATAACCTCTACACTACGGAAACGATCGTCGGACTGTAGA73380.12199523556900935No Hit
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT73290.12184560936021661Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC73130.12157960721125177Illumina RNA PCR Primer (100% over 32bp)
GGCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG72490.12051559861539231Illumina RNA PCR Primer (100% over 37bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA72290.12018309592918625No Hit
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT71140.11827120548350131Illumina RNA PCR Primer (100% over 39bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC70950.11795532793160554Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG69510.11556130859092179Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC69130.11492955348713023Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG69020.1147466770097169No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT69000.11471342674109629Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG66570.11067351910369246Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG65130.1082794997630087Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGTG63740.10596860609387648Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA63500.10556960287042919No Hit
ACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG63380.10537010125870555Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG63000.10473834615491401Illumina RNA PCR Primer (100% over 33bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC62170.10335846000715879Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT62170.10335846000715879Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA61260.10184557278492114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)