FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8193356
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA98196911.984942433845179No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA3050903.7236268020088468No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2912043.554148019444047Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2137562.6088943285266746No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1781102.173834506885823No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1680692.051283991565849No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1464611.7875581141598145No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1181861.4424614285037778Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1124381.372307025350784Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1085151.3244267672489758No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1048011.2790973564434402No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG992091.2108469350044109Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG900741.0993541596386145No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT869381.0610792451835365Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA861891.0519376919543104No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT715930.873793351588775Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT638400.7791679014069449No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG624340.7620076559592919No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA606380.7400874562267281No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT536640.6549697096037326Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG520550.6353318469257286No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA482100.5884035796809024No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA475650.5805313475943191No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG462920.5649943686079306No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT448200.5470285924351389Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC422080.5151491037372232No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT393990.4808652278748781Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG382440.46676844018494984Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA382170.46643890488830214No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC360650.44017372124438386Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG326610.398627863844803Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA316130.3858370123304785No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT309060.3772080695627042Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG305740.3731560059150365Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAAC303210.37006813813533795Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG301770.368310616553217Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA289750.35364019334690205No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG279320.34091036688751225Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC277820.33907961523946967Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC273760.3341243807787676No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT239610.2924442682583303Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA233260.28469408628161647No Hit
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC227990.27826204549149336Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG227430.2775785648762241Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT223810.27316035089894786Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA212190.25897812813211096No Hit
AAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAACG194060.236850443212769Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT191570.23381139547701818Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT191030.23315232488372284Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT186660.22781873508242534No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT183950.22451117710496163Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG182690.2229733457206058Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA177550.21669997007331304No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA174790.21333138704091462No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA171040.20875450792080802No Hit
CGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAAC160960.19645185684596156No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT158420.193351784055276Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT157310.19199702783572445No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA154970.18914105526477795No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT152050.1855771920565883Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAACT147030.17945027654113893No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA145350.1773998346953312No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA143240.17482457737708457No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT141600.17282295557522462Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT141590.17281075056423764Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG139990.17085794880632552Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC139310.17002800805921287No Hit
GCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA135280.16510938863147165No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC134390.16402314265363302Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA133330.16272941148901623No Hit
GCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAACT132790.1620703408957209No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT130090.15877498792924413Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC124200.15158623645793007No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG123020.15014604516146987Illumina DpnII expression Sequencing Primer (95% over 24bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA121680.14851057368921844No Hit
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT120800.14743653272236676Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGT120030.14649674687637154No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG116660.1423836581737691Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC115130.1405162914927656Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG107420.13110622802182645Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT105980.12934870643970556Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG105840.12917783628588822No Hit
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA104800.12790851514324533No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG102010.12450331707788602No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC101950.12443008701196433Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG101500.12388086151755154No Hit
ACTGAGGGGCCTCAGACCGAGCTGATCGTCGGACTGTAGAACTCTGAACG97920.11951146758422312Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT94860.11577673422221615Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG93800.11448300305759934Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG93660.11431213290378205Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC89510.10924705334419743Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA89020.10864900780583682No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG88300.10777024701477636No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA87750.10709897141049406No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG86980.10615918556449884Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA85880.10481663435593426No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA83450.10185081668610518Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT82790.10104528596096643Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA82640.10086221079616217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)