FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7633582
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA76808310.061894926916356No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2442313.1994285251668217Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2072642.7151604580916273No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1687002.20997167515853No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1419491.8595333095262487Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1168671.530958860466816Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1162981.5235049548167556No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT896461.1743634901675255Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT887021.1619970807937874Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT766121.0036179607424143Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA751710.9847408464335615No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA743370.9738154381521023No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC688660.9021452838261251No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT676650.8864121719004263Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG659610.8640897549800343No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT643330.8427629388143075Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG638460.8363832339784913No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA615690.8065545113683197Illumina RNA PCR Primer (100% over 45bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT610080.7992054057976976Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG563130.7377008591772513Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG562650.7370720587006205No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA539810.7071516360209401No Hit
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT428590.5614533255816209Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA427540.5600778245389909No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC423270.5544841202989632Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA409150.535986906278075No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA373580.48939017095774956No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA358530.4696746560133892No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG352370.4616050498966278Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC344330.45107264191306257Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA290010.3799133879743481Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG255350.3345087535576352Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT254810.3338013530214256Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT253950.3326747521674622Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG249170.3264129474210141No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT242650.3178717409467796Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA242020.3170464403212018No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC221840.2906106202828502No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT220500.28885521895225597Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT215790.2826851142753166Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG212020.2777464105317792Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG207830.27225750637118984Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC200090.26211809868551883No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA194730.255096493363142No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA191970.2514808906225151No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG176830.2316474755887865No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG175000.2292501737716317Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA172900.22649917168637212No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC166490.2181020653213655Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA166220.2177483650532607No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT164420.21539036326589533Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT163100.21366116195516074Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA159730.20924645860881563No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT155080.2031549539914551Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG154700.20265715361412243Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC153370.20091485229345804No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG145190.19019904417087546Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG144610.18943924359494663Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT142880.18717294187708994Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT142070.18611184107277554Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG141740.18567954074509188Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT137590.18024303662422175Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT137310.17987623634618716Illumina RNA PCR Primer (100% over 31bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG135750.17783263479713718No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG134920.17674533397296316Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA134650.17639163370485833No Hit
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG133370.17471483243384298Illumina RNA PCR Primer (100% over 34bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG127540.1670775266447652Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG126920.16626532602911712Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT123350.16158862248417585Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT123030.161169422166422No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC122750.1608026218883874Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG122560.16055372169972104Illumina RNA PCR Primer (100% over 40bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG121170.1587328203194778Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA115970.15192081515597788No Hit
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA115910.15184221509639903No Hit
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT115500.15130511468927693Illumina RNA PCR Primer (100% over 31bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT113330.1484624125345087No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT109670.14366780890019915Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT105890.1387160051467319Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG99740.13065949903990026Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT97430.12763339674611474Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT96470.1263757957928532Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC95070.12454179440268015Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA94300.12353309363808498No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT93870.12296979321110325Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG93410.1223671927543321Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT89620.11740228899093505Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG89440.11716648881219853Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT85560.1120836849594332Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT84870.11117978427427648Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA83190.10897898260606882No Hit
AGACAGCTACAGGTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT81950.10735458137477268Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGGGGCTCGTCCGGGATGATCGTCGGACTGTAGAACTCTGAACGTGTAG81380.10660788080877365Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA79730.1044463791703554No Hit
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT79290.10386997873344389Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT78050.10224557750214776No Hit
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA77890.10203597734327083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)