FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7831061
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA7478989.55040447265064No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3014063.8488526650475583Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1548461.9773310410939209No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1366101.7444634896854974No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG1303741.664831879102972No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1299321.659187688615885Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1275291.6285021914654987Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1241941.5859153695776345No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1049801.3405590889919003Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG949011.2118536683598813No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT923861.1797379690951202Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG849941.0853446295463667Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT578030.7381247572966166Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT535930.6843644813901973Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA509800.650997355275358No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA506330.6465662826531424No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA489900.6255857284217298No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT463150.59142688327929Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG446860.5706251043121743Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA446080.5696290706968059No Hit
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC445350.5686968853901151Illumina RNA PCR Primer (100% over 39bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG434250.5545225608637195Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG428990.5478057187908509Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA424160.5416379721726086No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT416980.5324693550465256Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT374970.47882400609572573Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC372090.4751463435159042Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT358110.457294356409687Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC344140.4394551389652054No Hit
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG337690.43121870714581334Illumina RNA PCR Primer (100% over 40bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA314080.4010695357883179No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC308700.39419945777462334Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC293000.37415108884990167Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA291150.37178870142883574No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA287110.36662975808769716No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG266360.34013270998655226No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA256610.327682289794448No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT243400.31081356664186377Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA240630.3072763703411326No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT238060.30399456727511126Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG229580.29316589412341443Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA217740.2780466146285925No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG217340.2775358281591728No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT213240.27230026684762126Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG209000.2668859302717729Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG195090.24912333079770416No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA191940.24510088735102434Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT187670.23964824178996946Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG187030.23883098343889803Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG186610.23829465764600735Illumina DpnII expression Sequencing Primer (95% over 24bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC184110.23510224221213447Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT175530.22414587244308273Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT174540.22288167593126906Illumina RNA PCR Primer (100% over 31bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC171580.2191018560575636Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT171070.21845060330905353Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT163260.20847749749363462Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG160140.20449336303216126Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT158350.20220759358150828Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC156990.20047091958548144No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG156780.20020275668903614No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTG155620.19872147592771913No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGT154880.19777652095929274No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG152480.19471180214277478Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG152320.1945074875550069Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC144300.1842662188431427Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG142450.18190383142207678Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA141990.18131642698224418No Hit
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG129470.16532881048940878Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA121200.1547683002341573No Hit
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT120940.1544362890290345Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT120130.1534019464284597Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC117140.14958381756954772No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT113590.14505058765344822Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA110940.14166662729354298No Hit
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC109350.13963625107759983Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA105890.13521794811711976No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG104650.13363451006191882Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT103570.1322553865944857Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTTCTGACTCCAAGTCCAGTGATCGTCGGACTGTAGAACTCTGAACGT103230.13182121809547903Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT100110.12783708363400567Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT99770.12740291513499896Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAAC98170.12535976925732029Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC97690.12474682549401671Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA96720.12350816830567404No Hit
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG96440.12315061777708027Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA95090.12142671344278892No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG94990.12129901682543401Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT94230.12032852253353664Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG93100.1188855507574261Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG91270.11654870265983114Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGGGGCTCGTCCGGGATGATCGTCGGACTGTAGAACTCTGAACGTGTAG89720.11456940509082995Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT89680.114518326443888Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA86830.11087897284927292No Hit
TCAGTGGCTCAGGTTCGTTGCAGATCGTCGGACTGTAGAACTCTGAACGT86210.11008725382167243Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT84920.10843996745779404Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG83970.10722684959292234Illumina RNA PCR Primer (100% over 36bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA82460.10529863067086312No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA81050.10349810836615882Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG79070.10096971534253149Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG78330.10002476037410513Illumina DpnII expression Sequencing Primer (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)