FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003293717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7585376
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA118203315.583050859970554No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA5157256.7989378509384375No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA2155882.84215311146079No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2061962.71833591373717Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1743082.2979480516193265No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1637822.1591810346645968Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1553912.048560282311648Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1553562.0480988681378482No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1166471.5377879751774994No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1041441.3729576490341415No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT999101.3171397172664876Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA983301.2963101631349587No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA826261.0892802149820917No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG665370.8771747109174284No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA628050.8279747767282729No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT538490.7099054812839865Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA473270.6239242458119413No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA438160.577637812548778No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG427030.562964841821948Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT420110.5538420244428226Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA419120.552536881494075No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA391740.5164411098408306No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA383520.5056044683875921No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG349460.46070227764582794Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT330850.436168226861793Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA321480.4238155102660699No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC318480.4198605316335011No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA303850.40057341916867406No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC298870.39400815463860983No Hit
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG276640.36470176297127527Illumina DpnII expression Sequencing Primer (96% over 29bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT270200.35621174217336093No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT264120.3481963188113549Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC258500.3407873255063427Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG230370.3037028091949562No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG228160.3007893082689639No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA209970.2768089544934885No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA202450.2668951413878495No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTA198900.2622150833393097No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG197910.26090994039056203Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG192930.25434467586049786No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC190440.2510620435954658No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT185960.24515594217082975Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT166290.21922446560328715Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA156330.20609393654315886No Hit
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA154620.20383959872259466No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA152990.20169072699889892No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA149830.19752481617259315No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG144510.19051132073083787Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT141160.1860949279244694Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG136480.17992516125766211Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC135700.17889686681319422Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT135430.17854091873626304No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGT135320.17839590285306886No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT134010.17666889551684717Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA129800.17111874216914233No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG128820.16982678248250316Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT126850.16722967984711634Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT125460.16539720641402617No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG124690.16438209523166683No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG123790.1631956016418962Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA121250.1598470530663213No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT119390.15739496631412864Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG111410.14687472315149572Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG106370.14023035904878017Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG105510.1390965985074438Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA104060.13718502550170222No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA101900.1343374408862527No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT100930.13305866446172213Illumina RNA PCR Primer (100% over 31bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC100590.13261043355003102No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA100520.13251815071527107No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG99250.13084387642748363Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT99100.1306461274958552No Hit
AGGCGAATGTGATAACCGCTACACTACGGAAACGATCGTCGGACTGTAGA98140.12938053433343316No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG95270.1255969381082757Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTTAACAATAACCCACAACACTCGGACCAACTGATCGTCGGACTGTAG94190.12417314580055097No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAGA94070.1240149466552482No Hit
TCACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACG94020.12394903034470539Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG90870.11979630278050818Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT89020.1173573992904241Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA88620.1168300688060816No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT87440.11527444387727122Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG87130.11486576275190577Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC86230.11367926916213512Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG85780.11308602236724984No Hit
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG85130.11222911033019327Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTTAACAATAACCCACAACACTCGGACCAACTGATCGTCGGACTGTAGA85110.11220274380597613No Hit
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG84160.1109503339056627Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG82600.10889374501672693No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG82070.10819503212497311No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT82000.10810274929021317Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC79240.10446416894824989Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGGACTGTAG78830.10392365520179882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)